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  • Western Blot (WB) analysis of pig cartilage tissue using Decorin Monoclonal antibody (66847-1-Ig)

    WB analysis of pig cartilage using 66847-1-Ig

    pig cartilage tissue were subjected to SDS PAGE followed by western blot with 66847-1-Ig (Decorin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of BxPC-3 cells using Decorin Monoclonal antibody (66847-1-Ig)

    IF Staining of BxPC-3 using 66847-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using Decorin antibody (66847-1-Ig, Clone: 3A4E4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

Decorin Monoclonal antibody

Decorin Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66847-1-Ig
Clone No.3A4E4

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF/ICC, ELISA

Synonyms

DCN, PG40, PG S2, DSPG2, CSCD

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inpig cartilage tissue
Positive IF/ICC detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66847-1-Ig targets Decorin in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Decorin fusion protein Ag6275 Predict reactive species
Full Name decorin
Calculated Molecular Weight 359 aa, 40 kDa
Observed Molecular Weight 40 kDa, 100 kDa
GenBank Accession NumberBC005322
Gene Symbol Decorin
Gene ID (NCBI) 1634
RRIDAB_2882187
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07585
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis.

Protocols

Product Specific Protocols
WB protocol for Decorin antibody 66847-1-IgDownload protocol
IF protocol for Decorin antibody 66847-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols