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FNTB Monoclonal antibody

FNTB Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1F3B10

Cat no : 66783-1-Ig

Synonyms

FTase-beta, FTase beta, FPTB, EC:2.5.1.58, 1F3B10

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FNTB Monoclonal antibody (66783-1-Ig)

    WB analysis of HeLa using 66783-1-Ig

    WB result of FNTB antibody (66783-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FNTB transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using FNTB Monoclonal antibody (66783-1-Ig)

    WB analysis using 66783-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FNTB Monoclonal antibody (66783-1-Ig)

    WB analysis of HEK-293 using 66783-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FNTB Monoclonal antibody (66783-1-Ig)

    WB analysis using 66783-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FNTB Monoclonal antibody (66783-1-Ig)

    IF Staining of A431 using 66783-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FNTB antibody (66783-1-Ig, Clone: 1F3B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of A431 cells using FNTB Monoclonal antibody (66783-1-Ig)

    FC experiment of A431 using 66783-1-Ig

    1x10^6 A431 cells were intracellularly stained with 0.8 ug FNTB Monoclonal antibody (66783-1-Ig, Clone:1F3B10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inNIH/3T3 cells, HEK-293 cells, HepG2 cells, HeLa cells, K-562 cells, 4T1 cells
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66783-1-Ig targets FNTB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FNTB fusion protein Ag27796
Full Name farnesyltransferase, CAAX box, beta
Calculated Molecular Weight 437 aa, 49 kDa
Observed Molecular Weight 44-45 kDa
GenBank Accession NumberBC020232
Gene Symbol FNTB
Gene ID (NCBI) 2342
RRIDAB_2882128
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.

Protocols

Product Specific Protocols
WB protocol for FNTB antibody 66783-1-IgDownload protocol
IF protocol for FNTB antibody 66783-1-IgDownload protocol
FC protocol for FNTB antibody 66783-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols