FNTB Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

FNTB Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1F3B10

Cat no : 66783-1-PBS

Synonyms

FTase-beta, FTase beta, FPTB, EC:2.5.1.58, 1F3B10

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Validation Data Gallery

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  WB analysis using 66783-1-Ig (same clone as 66783-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66783-1-Ig (same clone as 66783-1-PBS)

  WB result of FNTB antibody (66783-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FNTB transfected HeLa cells. This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66783-1-Ig (same clone as 66783-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66783-1-Ig (same clone as 66783-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66783-1-Ig (FNTB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66783-1-Ig (same clone as 66783-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FNTB antibody (66783-1-Ig, Clone: 1F3B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 66783-1-Ig (same clone as 66783-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.8 ug FNTB Monoclonal antibody (66783-1-Ig, Clone:1F3B10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66783-1-PBS in a different storage buffer formulation.

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Product Information

66783-1-PBS targets FNTB in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FNTB fusion protein Ag27796
• Full Name: farnesyltransferase, CAAX box, beta
• Calculated Molecular Weight: 437 aa, 49 kDa
• Observed Molecular Weight: 44-45 kDa
• GenBank Accession Number: BC020232
• Gene Symbol: FNTB
• Gene ID (NCBI): 2342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.