CoraLite®594-conjugated FNTB Monoclonal antibody

• Featured Product
• KD/KO Validated

FNTB Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F3B10

Cat no : CL594-66783

Synonyms

FTase-beta, FTase beta, FPTB, EC:2.5.1.58, 1F3B10

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Validation Data Gallery

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  IF Staining of A431 using CL594-66783

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 FNTB antibody (CL594-66783, Clone: 1F3B10 ) at dilution of 1:400.

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  IF Staining of A431 using CL594-66783

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 FNTB antibody (CL594-66783, Clone: 1F3B10 ) at dilution of 1:400.

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  FC experiment of A431 using CL594-66783

  1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human FNTB (CL594-66783, Clone:1F3B10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: A431 cells
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66783 targets FNTB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FNTB fusion protein Ag27796
• Full Name: farnesyltransferase, CAAX box, beta
• Calculated Molecular Weight: 437 aa, 49 kDa
• Observed Molecular Weight: 44-45 kDa
• GenBank Accession Number: BC020232
• Gene Symbol: FNTB
• Gene ID (NCBI): 2342
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.