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BAG6 Monoclonal antibody

BAG6 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B8D3

Cat no : 66661-1-Ig

Synonyms

BAG 6, BAG6, BAT3, D6S52E, G3, HLA B associated transcript 3, Protein G3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using BAG6 Monoclonal antibody (66661-1-Ig)

    WB analysis using 66661-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of HEK-293 cells using BAG6 Monoclonal antibody (66661-1-Ig)

    WB analysis of HEK-293 using 66661-1-Ig

    WB result of BAG6 antibody (66661-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BAG6 transfected HEK-293 cells.

  • Western Blot (WB) analysis of HeLa cells using BAG6 Monoclonal antibody (66661-1-Ig)

    WB analysis of HeLa using 66661-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using BAG6 Monoclonal antibody (66661-1-Ig)

    WB analysis of HSC-T6 using 66661-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using BAG6 Monoclonal antibody (66661-1-Ig)

    WB analysis of NIH/3T3 using 66661-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66661-1-Ig (BAG6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BAG6 Monoclonal antibody (66661-1-Ig)

    IHC staining of human breast cancer using 66661-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using BAG6 Monoclonal antibody (66661-1-Ig)

    IHC staining of human breast cancer using 66661-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using BAG6 Monoclonal antibody (66661-1-Ig)

    IHC staining of human testis using 66661-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using BAG6 Monoclonal antibody (66661-1-Ig)

    IHC staining of human testis using 66661-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66661-1-Ig (BAG6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, HSC-T6 cells, NIH/3T3 cells, RAW 274.7 cells, PC-12 cells, Jurkat cells, HepG2 cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66661-1-Ig targets BAG6 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BAG6 fusion protein Ag24525
Full Name HLA-B associated transcript 3
Calculated Molecular Weight 119 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC003133
Gene Symbol BAT3/BAG-6
Gene ID (NCBI) 7917
RRIDAB_2858188
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BAT3 also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. BAT3 was first discovered as a member of a group of genes located within the class III region of the human major histocompatibility complex on chromosome 6, and has been extensively studied for its role in regulating apoptosis under various stress conditions such as DNA damage and endoplasmic reticulum-related stress. BAT3 has been shown to be required for p53 acetylation, which is critical for the enhancement of p53 transcriptional activity in response to DNA damage. In addition, BAT3 is involved in the regulation of development and reproduction of mammals by acting as a co-chaperone of the heat shock protein HSP70.

Protocols

Product Specific Protocols
WB protocol for BAG6 antibody 66661-1-IgDownload protocol
IHC protocol for BAG6 antibody 66661-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

RNF126-Mediated Reubiquitination Is Required for Proteasomal Degradation of p97-Extracted Membrane Proteins.

Authors - Xianyan Hu
View Article