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CoraLite® Plus 488-conjugated BAG6 Monoclonal antibody
BAG6 Monoclonal Antibody for WB, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B8D3
Cat no : CL488-66661
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, LNCaP cells, HepG2 cells, NIH/3T3 cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66661 targets BAG6 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | BAG6 fusion protein Ag24525 |
Full Name | HLA-B associated transcript 3 |
Calculated Molecular Weight | 119 kDa |
Observed Molecular Weight | 150-160 kDa |
GenBank Accession Number | BC003133 |
Gene Symbol | BAT3/BAG-6 |
Gene ID (NCBI) | 7917 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
BAT3 also known as Scythe or BAG6, is a nuclear protein implicated in the control of apoptosis and natural killer (NK) cell-dendritic cell (DC) interaction. BAT3 was first discovered as a member of a group of genes located within the class III region of the human major histocompatibility complex on chromosome 6, and has been extensively studied for its role in regulating apoptosis under various stress conditions such as DNA damage and endoplasmic reticulum-related stress. BAT3 has been shown to be required for p53 acetylation, which is critical for the enhancement of p53 transcriptional activity in response to DNA damage. In addition, BAT3 is involved in the regulation of development and reproduction of mammals by acting as a co-chaperone of the heat shock protein HSP70.
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 488 BAG6 antibody CL488-66661 | Download protocol |
FC protocol for CL Plus 488 BAG6 antibody CL488-66661 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |