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  • Featured Product
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NSUN2 Monoclonal antibody

NSUN2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2H5A2

Cat no : 66580-1-Ig

Synonyms

hTrm4, MISU, NSUN2, SAKI, TRM4

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using NSUN2 Monoclonal antibody (66580-1-Ig)

    WB analysis of COLO 320 using 66580-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66580-1-Ig (NSUN2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NSUN2 Monoclonal antibody (66580-1-Ig)

    WB analysis of HepG2 using 66580-1-Ig

    WB result of NSUN2 antibody (66580-1-Ig; 1:40000; room temperature for 1.5 hours) with negative control and NSUN2 overexpressed HepG2 cells.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NSUN2 Monoclonal antibody (66580-1-Ig)

    IP experiment of HepG2 using 66580-1-Ig

    IP result of anti-NSUN2 (IP:66580-1-Ig, 4ug; Detection:66580-1-Ig 1:4000) with HepG2 cells lysate 1920 ug.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using NSUN2 Monoclonal antibody (66580-1-Ig)

    IHC staining of human oesophagus cancer using 66580-1-Ig

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NSUN2 Monoclonal antibody (66580-1-Ig)

    IHC staining of human breast cancer using 66580-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NSUN2 Monoclonal antibody (66580-1-Ig)

    IHC staining of human breast cancer using 66580-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NSUN2 Monoclonal antibody (66580-1-Ig)

    IF Staining of HepG2 using 66580-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66580-1-Ig (NSUN2 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inCOLO 320 cells, HepG2 cells, Caco-2 cells, HEK-293 cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman oesophagus cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66580-1-Ig targets NSUN2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NSUN2 fusion protein Ag14791
Full Name NOL1/NOP2/Sun domain family, member 2
Calculated Molecular Weight 767 aa, 86 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC001041
Gene Symbol NSUN2
Gene ID (NCBI) 54888
RRIDAB_2881940
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NSUN2 antibody 66580-1-IgDownload protocol
IHC protocol for NSUN2 antibody 66580-1-IgDownload protocol
IF protocol for NSUN2 antibody 66580-1-IgDownload protocol
IP protocol for NSUN2 antibody 66580-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

N6-methyladenosine modification of circNSUN2 facilitates cytoplasmic export and stabilizes HMGA2 to promote colorectal liver metastasis.

Authors - Ri-Xin Chen
View Article
human,IF

Acta Neuropathol

RNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation

Authors - Yoon A Kim
View Article
humanWB,CoIP

Cell Metab

NSUN2 is a glucose sensor suppressing cGAS/STING to maintain tumorigenesis and immunotherapy resistance

Authors - Tingjin Chen
View Article
humanWB

Virol Sin

NSUN2 mediates distinct pathways to regulate enterovirus 71 replication

Authors - Lishi Liu
View Article