CoraLite® Plus 488-conjugated NSUN2 Monoclonal antibody

Name: CoraLite® Plus 488-conjugated NSUN2 Monoclonal antibody, 100ul
Brand: Proteintech
SKU: CL488-66580-100UL

NSUN2 Monoclonal Antibody for IF/ICC

Cat No. CL488-66580

Clone No. 2H5A2

Publications (1)

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Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

IF/ICC

Synonyms

FLJ20303, hTrm4, MISU, NSUN2, SAKI, TRM4

Formulation: PBS, Proclin300, BSA, Glycerol

PBS, Proclin300, BSA, Glycerol

Conjugate: CoraLite® Plus 488

Unconjugated CoraLite® Plus 488

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Filter: AllIF/ICC

IF Staining of HepG2 using CL488-66580
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66580 (NSUN2 antibody) at dilution of 1:100.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for NSUN2 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive IF/ICC detected in

HepG2 cells

Recommended dilution

Application	Dilution
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66580 targets NSUN2 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity	Human
Cited Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag14791 Product name: Recombinant human NSUN2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 415-767 aa of BC001041 Sequence: HHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR Predict reactive species
Full Name	NOL1/NOP2/Sun domain family, member 2
Calculated Molecular Weight	767 aa, 86 kDa
Observed Molecular Weight	100 kDa
GenBank Accession Number	BC001041
Gene Symbol	NSUN2
Gene ID (NCBI)	54888
RRID	AB_2883358
Conjugate	CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths	493 nm / 522 nm
Excitation Laser	Blue laser (488 nm)
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q08J23
Storage Buffer	PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage Conditions	Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 NSUN2 antibody CL488-66580	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The NSUN2 Monoclonal antibody (2H5A2), catalog number CL488-66580 (CoraLite® Plus 488 conjugate), has 1 citation. It appears in Nucleic Acids Research (IF 15, 2024). The cited study investigates spatial regulation of NSUN2-mediated tRNA m5C installation in cognitive function, placing it in the fields of RNA modification and neuroscience. The application used was immunofluorescence (IF) in human samples.