NSUN2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

NSUN2 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H5A2

Cat no : 66580-1-PBS

Synonyms

hTrm4, MISU, NSUN2, SAKI, TRM4

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Validation Data Gallery

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  WB analysis of COLO 320 using 66580-1-Ig (same clone as 66580-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66580-1-Ig (NSUN2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

  WB result of NSUN2 antibody (66580-1-Ig; 1:40000; room temperature for 1.5 hours) with negative control and NSUN2 overexpressed HepG2 cells. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

  IP result of anti-NSUN2 (IP:66580-1-Ig, 4ug; Detection:66580-1-Ig 1:4000) with HepG2 cells lysate 1920 ug. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 66580-1-Ig (same clone as 66580-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66580-1-Ig (same clone as 66580-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66580-1-Ig (same clone as 66580-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66580-1-Ig (NSUN2 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66580-1-PBS targets NSUN2 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: NSUN2 fusion protein Ag14791
• Full Name: NOL1/NOP2/Sun domain family, member 2
• Calculated Molecular Weight: 767 aa, 86 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC001041
• Gene Symbol: NSUN2
• Gene ID (NCBI): 54888
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.