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SP1 Monoclonal antibody

SP1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

4G3H3

Cat no : 66508-1-Ig

Synonyms

TSFP1, Specificity protein 1, Sp1 transcription factor, SP 1, 4G3H3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SP1 Monoclonal antibody (66508-1-Ig)

    WB analysis using 66508-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 66508-1-Ig (SP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using SP1 Monoclonal antibody (66508-1-Ig)

    WB analysis of NIH/3T3 using 66508-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66508-1-Ig (SP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SP1 Monoclonal antibody (66508-1-Ig)

    IHC staining of human lung cancer using 66508-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SP1 Monoclonal antibody (66508-1-Ig)

    IHC staining of human colon cancer using 66508-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SP1 Monoclonal antibody (66508-1-Ig)

    IHC staining of human colon cancer using 66508-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using SP1 Monoclonal antibody (66508-1-Ig)

    IHC staining of human lung cancer using 66508-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66508-1-Ig (SP1 antibody) at dilution of 1:350 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using SP1 Monoclonal antibody (66508-1-Ig)

    IF Staining of human colon cancer using 66508-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using SP1 antibody (66508-1-Ig, Clone: 4G3H3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SP1 Monoclonal antibody (66508-1-Ig)

    IF Staining of HEK-293 using 66508-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SP1 antibody (66508-1-Ig, Clone: 4G3H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HEK-293 cells using SP1 Monoclonal antibody (66508-1-Ig)

    FC experiment of HEK-293 using 66508-1-Ig

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human SP1 (66508-1-Ig, Clone:4G3H3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inJurkat cells, NIH/3T3 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:175-1:700
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66508-1-Ig targets SP1 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SP1 fusion protein Ag16720
Full Name Sp1 transcription factor
Calculated Molecular Weight 785 aa, 81 kDa
Observed Molecular Weight 95-105 kDa, 60-65 kDa
GenBank Accession NumberBC062539
Gene Symbol SP1
Gene ID (NCBI) 6667
RRIDAB_2881871
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].

Protocols

Product Specific Protocols
WB protocol for SP1 antibody 66508-1-IgDownload protocol
IHC protocol for SP1 antibody 66508-1-IgDownload protocol
IF protocol for SP1 antibody 66508-1-IgDownload protocol
FC protocol for SP1 antibody 66508-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Br J Pharmacol

Sarco/endoplasmic reticulum Ca 2+ -ATPase 2b mediates oxidation-induced endoplasmic reticulum stress to regulate neuropathic pain

Authors - Shaoheng Li
View Article
mouseIHC

Evid Based Complement Alternat Med

Guifu Dihuang Pills Ameliorated Mucus Hypersecretion by Suppressing Muc5ac Expression and Inactivating the ERK-SP1 Pathway in Lipopolysaccharide/Cigarette Smoke-Induced Mice

Authors - Huanhuan Zhang
View Article
humanWB

Endocrinology

AURKA enhances the glycolysis and development of ovarian endometriosis through ERβ

Authors - Yujun Sun
View Article
humanWB

Acta Biochim Biophys Sin (Shanghai)

Propofol protects against high glucose-mediated endothelial injury via inhibition of COX2 and iNOS expressions

Authors - Jiayun Shao
View Article
humanWB

Genomics

STRIP2 is regulated by the transcription factor Sp1 and promotes lung adenocarcinoma progression via activating the PI3K/AKT/mTOR/MYC signaling pathway

Authors - Junfan Pan
View Article