CoraLite®594-conjugated SP1 Monoclonal antibody

SP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4G3H3

Cat no : CL594-66508

Synonyms

TSFP1, Specificity protein 1, Sp1 transcription factor, SP 1, 4G3H3

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Validation Data Gallery

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  IF Staining of human colon cancer using CL594-66508

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 SP1 antibody (CL594-66508, Clone: 4G3H3 ) at dilution of 1:200.

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  IF Staining of HEK-293 using CL594-66508

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 SP1 antibody (CL594-66508, Clone: 4G3H3 ) at dilution of 1:200.

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  FC experiment of HEK-293 using CL594-66508

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SP1 (CL594-66508, Clone:4G3H3) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: human colon cancer tissue
• Positive IF/ICC detected in: HEK-293 cells, human colon cancer tissue
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66508 targets SP1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SP1 fusion protein Ag16720
• Full Name: Sp1 transcription factor
• Calculated Molecular Weight: 785 aa, 81 kDa
• Observed Molecular Weight: 95-105 kDa, 60-65 kDa
• GenBank Accession Number: BC062539
• Gene Symbol: SP1
• Gene ID (NCBI): 6667
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].