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  • Featured Product
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CCAR2 Monoclonal antibody

CCAR2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2D10F8

Cat no : 66497-1-Ig

Synonyms

cell cycle and apoptosis regulator 2, DBC1, DBC-1, KIAA1967, DBC.1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CCAR2 Monoclonal antibody (66497-1-Ig)

    WB analysis using 66497-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66497-1-Ig (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CCAR2 Monoclonal antibody (66497-1-Ig)

    WB analysis of HEK-293 using 66497-1-Ig

    WB result of CCAR2 antibody (66497-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCAR2 transfected HEK-293 cells.

  • Western Blot (WB) analysis of pig brain tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    WB analysis of pig brain using 66497-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66497-1-Ig (CCAR2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human cervical cancer using 66497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human cervical cancer using 66497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human colon cancer using 66497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human colon cancer using 66497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human colon cancer using 66497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human colon cancer using 66497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human colon using 66497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human colon using 66497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human lung cancer using 66497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CCAR2 Monoclonal antibody (66497-1-Ig)

    IHC staining of human lung cancer using 66497-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66497-1-Ig (CCAR2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCAR2 Monoclonal antibody (66497-1-Ig)

    IF Staining of HeLa using 66497-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66497-1-Ig (CCAR2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CCAR2 Monoclonal antibody (66497-1-Ig)

    IF Staining of HeLa using 66497-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CCAR2 antibody (66497-1-Ig, Clone: 2D10F8 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of U2OS cells using CCAR2 Monoclonal antibody (66497-1-Ig)

    FC experiment of U2OS using 66497-1-Ig

    1X10^6 U2OS cells were intracellularly stained with 0.2 ug Anti-Human CCAR2 (66497-1-Ig, Clone:2D10F8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHeLa cells, pig brain tissue, HEK-293 cells, MCF-7 cells, Jurkat cells
Positive IHC detected inhuman cervical cancer tissue, human colon tissue, human lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66497-1-Ig targets CCAR2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CCAR2 fusion protein Ag18374
Full Name KIAA1967
Calculated Molecular Weight 923 aa, 103 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC018269
Gene Symbol CCAR2
Gene ID (NCBI) 57805
RRIDAB_2881861
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cell cycle and apoptosis regulator protein 2 (CCAR2), previously known as DBC1/KIAA1967, regulates diverse cellular functions including transcription, mRNA splicing, metabolism, and circadian cycle. CCAR2 is a critical regulator of cell death or survival following cellular stresses. CCAR2 is a widely expressed protein involved in the regulation of a variety of transcriptional complexes. High expression of CCAR2 correlates with poor outcomes in many human tumor types such as squamous cell carcinoma (SCC).

Protocols

Product Specific Protocols
WB protocol for CCAR2 antibody 66497-1-IgDownload protocol
IHC protocol for CCAR2 antibody 66497-1-IgDownload protocol
IF protocol for CCAR2 antibody 66497-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols