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PR Monoclonal antibody

PR Monoclonal Antibody for IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D1D12

Cat no : 66300-1-Ig

Synonyms

NR3C3, PGR, PR, progesterone receptor

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Monoclonal antibody (66300-1-Ig)

    IHC staining of human breast cancer using 66300-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66300-1-Ig (PR antibody) at dilution of 1:9900 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Monoclonal antibody (66300-1-Ig)

    IHC staining of human breast cancer using 66300-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66300-1-Ig (PR antibody) at dilution of 1:9900 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PR Monoclonal antibody (66300-1-Ig)

    IHC staining of human breast cancer using 66300-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66300-1-Ig (PR antibody) at dilution of 1:3200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:5000-1:15000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 2 publications below

Product Information

66300-1-Ig targets PR in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PR fusion protein Ag22992
Full Name progesterone receptor
Calculated Molecular Weight 933 aa, 99 kDa
GenBank Accession NumberNM_000926
Gene Symbol Progesterone Receptor
Gene ID (NCBI) 5241
RRIDAB_2881683
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.

Protocols

Product Specific Protocols
IHC protocol for PR antibody 66300-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Ann Med

Organoids from patient biopsy samples can predict the response of BC patients to neoadjuvant chemotherapy

Authors - Dan Shu
View Article
humanIHC

Mol Med Rep

HPK1 positive expression associated with longer overall survival in patients with estrogen receptor-positive invasive ductal carcinoma‑not otherwise specified.

Authors - Jiaojiao Wang
View Article