Ceruloplasmin Monoclonal antibody
Ceruloplasmin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
3C8A5
Cat no : 66156-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | human placenta tissue, rat liver tissue, HSC-T6 cells, human kidney tissue, human blood tissue |
| Positive IHC detected in | human ovary tumor tissue, human placenta tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
Product Information
66156-1-Ig targets Ceruloplasmin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Ceruloplasmin fusion protein Ag15535 |
| Full Name | ceruloplasmin (ferroxidase) |
| Calculated Molecular Weight | 1065 aa, 122 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC146663 |
| Gene Symbol | Ceruloplasmin |
| Gene ID (NCBI) | 1356 |
| RRID | AB_2881552 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Ceruloplasmin antibody 66156-1-Ig | Download protocol |
| IHC protocol for Ceruloplasmin antibody 66156-1-Ig | Download protocol |
| IF protocol for Ceruloplasmin antibody 66156-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest. | ||
FEBS Open Bio Ceruloplasmin overexpression is associated with oncogenic pathways and poorer survival rates in clear-cell renal cell carcinoma. | ||
Clin Immunol Proteomic profiling of aqueous humor-derived exosomes in Vogt-Koyanagi-Harada disease and Behcet's uveitis | ||
Arch Toxicol Arylacetamide deacetylase regulates hepatic iron homeostasis to protect against carbon tetrachloride-induced ferroptosis |
