CoraLite®594-conjugated Ceruloplasmin Monoclonal antibody

Ceruloplasmin Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C8A5

Cat no : CL594-66156

Synonyms

Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66156

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 Ceruloplasmin antibody (CL594-66156, Clone: 3C8A5 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66156 targets Ceruloplasmin in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Ceruloplasmin fusion protein Ag15535
• Full Name: ceruloplasmin (ferroxidase)
• Calculated Molecular Weight: 1065 aa, 122 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC146663
• Gene Symbol: Ceruloplasmin
• Gene ID (NCBI): 1356
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.