Ceruloplasmin Monoclonal antibody, PBS Only

Ceruloplasmin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C8A5

Cat no : 66156-1-PBS

Synonyms

Ceruloplasmin, ceruloplasmin (ferroxidase), CP, CP 2, Ferroxidase

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Validation Data Gallery

• 

  WB analysis of rat liver using 66156-1-Ig (same clone as 66156-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66156-1-Ig (same clone as 66156-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human blood using 66156-1-Ig (same clone as 66156-1-PBS)

  human blood was subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 66156-1-Ig (same clone as 66156-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 66156-1-Ig (same clone as 66156-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66156-1-Ig (Ceruloplasmin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66156-1-Ig (Ceruloplasmin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66156-1-Ig (same clone as 66156-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ceruloplasmin antibody (66156-1-Ig, Clone: 3C8A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66156-1-PBS in a different storage buffer formulation.

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Product Information

66156-1-PBS targets Ceruloplasmin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Ceruloplasmin fusion protein Ag15535
• Full Name: ceruloplasmin (ferroxidase)
• Calculated Molecular Weight: 1065 aa, 122 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC146663
• Gene Symbol: Ceruloplasmin
• Gene ID (NCBI): 1356
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Ceruloplasmin (CP) is a serum glycoprotein synthesized by hepatocytes and functions as the major transport protein of copper in blood where it acts as ferrodoxidase and is required for iron transport of transferrin. The intact ceruloplasmin migrated around 130-140 kDa and some proteolytic cleaved fragments could also be observed.