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ORM1/2 Monoclonal antibody

ORM1/2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

5F2H7

Cat no : 66097-1-Ig

Synonyms

AGP 1, AGP A, AGP1, Alpha 1 acid glycoprotein 1, OMD 1, ORM, ORM1, ORM1/2, orosomucoid 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ORM1/2 Monoclonal antibody (66097-1-Ig)

    WB analysis using 66097-1-Ig

    Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • Western Blot (WB) analysis of various lysates using ORM1/2 Monoclonal antibody (66097-1-Ig)

    WB analysis using 66097-1-Ig

    Untreated and PNGase F-treated lysates of HuH-7 cells were subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • Western Blot (WB) analysis of human plasma using ORM1/2 Monoclonal antibody (66097-1-Ig)

    WB analysis of human plasma using 66097-1-Ig

    0.8μL human plasma were subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using ORM1/2 Monoclonal antibody (66097-1-Ig)

    WB analysis of human testis using 66097-1-Ig

    25μg human testis tissue was subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ORM1/2 Monoclonal antibody (66097-1-Ig)

    IHC staining of human liver cancer using 66097-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ORM1/2 Monoclonal antibody (66097-1-Ig)

    IHC staining of human liver cancer using 66097-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ORM1/2 Monoclonal antibody (66097-1-Ig)

    IHC staining of human liver using 66097-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ORM1/2 Monoclonal antibody (66097-1-Ig)

    IHC staining of human liver using 66097-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ORM1/2 Monoclonal antibody (66097-1-Ig)

    IHC staining of human liver cancer using 66097-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66097-1-Ig (ORM1/2 antibody at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ORM1/2 Monoclonal antibody (66097-1-Ig)

    IHC staining of human liver cancer using 66097-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66097-1-Ig (ORM1/2 antibody at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ORM1/2 Monoclonal antibody (66097-1-Ig)

    IF Staining of HepG2 using 66097-1-Ig

    Immunofluorescent analysis of () fixed HepG2 cells using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:25.

  • Flow cytometry (FC) experiment of HepG2 cells using ORM1/2 Monoclonal antibody (66097-1-Ig)

    FC experiment of HepG2 using 66097-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human ORM1/2 (66097-1-Ig, Clone:5F2H7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inhuman plasma tissue, HuH-7 cells, human testis tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66097-1-Ig targets ORM1/2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ORM1/2 fusion protein Ag19248
Full Name orosomucoid 1
Calculated Molecular Weight 201 aa, 24 kDa
Observed Molecular Weight 40-47 kDa
GenBank Accession NumberBC026238
Gene Symbol ORM1
Gene ID (NCBI) 5004
RRIDAB_2881496
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha-1-acid glycoprotein 1 (AGP1), also called orosomucoid-1 (ORM1), is a glycoprotein synthesized mostly by hepatocytes and present in human plasma. ORM1 is an acute-phase reactant protein controlled by glucocorticoids, interleukin-1 and interleukin-6, and increase up to 5-50 times upon infection and/or inflammation. Anti-apoptotic effect and role as immunomodulator of ORM have been reported. ORM is an important carrier for synthetic drugs and influences their distribution and availability in the body. This antibody recognizes a band about 44 kDa in human plasma which may be due to the glycosylation of ORM1 or the dimer formation of the protein. This antibody recognizes both ORM1 and ORM2.

Protocols

Product Specific Protocols
WB protocol for ORM1/2 antibody 66097-1-IgDownload protocol
IHC protocol for ORM1/2 antibody 66097-1-IgDownload protocol
IF protocol for ORM1/2 antibody 66097-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Bioengineered

Orosomucoid 1 promotes epirubicin resistance in breast cancer by upregulating the expression of matrix metalloproteinases 2 and 9.

Authors - Luo Qiong
View Article