CoraLite® Plus 488-conjugated ORM1/2 Monoclonal antibody

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  IF Staining of mouse liver using CL488-66097

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 ORM1/2 antibody (CL488-66097, Clone: 5F2H7 ) at dilution of 1:200.

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  IF Staining of HepG2 using CL488-66097

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66097 (ORM1/2 antibody) at dilution of 1:50.

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  FC experiment of HepG2 using CL488-66097

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ORM1/2 (CL488-66097, Clone:5F2H7) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

ORM1/2 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Cat No. CL488-66097

Clone No.5F2H7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, IF-P, FC (Intra)

Synonyms

AGP 1, AGP A, AGP1, Alpha 1 acid glycoprotein 1, OMD 1, ORM, ORM1, ORM1/2, orosomucoid 1

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Tested Applications

• Positive IF-P detected in: mouse liver tissue
• Positive IF/ICC detected in: HepG2 cells, mouse liver tissue
• Positive FC (Intra) detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66097 targets ORM1/2 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ORM1/2 fusion protein Ag19248
• Full Name: orosomucoid 1
• Calculated Molecular Weight: 201 aa, 24 kDa
• Observed Molecular Weight: 40-47 kDa
• GenBank Accession Number: BC026238
• Gene Symbol: ORM1
• Gene ID (NCBI): 5004
• RRID: AB_2883258
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P02763
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Alpha-1-acid glycoprotein 1 (AGP1), also called orosomucoid-1 (ORM1), is a glycoprotein synthesized mostly by hepatocytes and present in human plasma. ORM1 is an acute-phase reactant protein controlled by glucocorticoids, interleukin-1 and interleukin-6, and increase up to 5-50 times upon infection and/or inflammation. Anti-apoptotic effect and role as immunomodulator of ORM have been reported. ORM is an important carrier for synthetic drugs and influences their distribution and availability in the body. This antibody recognizes a band about 44 kDa in human plasma which may be due to the glycosylation of ORM1 or the dimer formation of the protein. This antibody recognizes both ORM1 and ORM2.