ORM1/2 Monoclonal antibody, PBS Only

ORM1/2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5F2H7

Cat no : 66097-1-PBS

Synonyms

AGP 1, AGP A, AGP1, Alpha 1 acid glycoprotein 1, OMD 1, ORM, ORM1, ORM1/2, orosomucoid 1

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Validation Data Gallery

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  WB analysis using 66097-1-Ig (same clone as 66097-1-PBS)

  Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66097-1-PBS in a different storage buffer formulation.

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  WB analysis using 66097-1-Ig (same clone as 66097-1-PBS)

  Untreated and PNGase F-treated lysates of HuH-7 cells were subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66097-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 66097-1-Ig (same clone as 66097-1-PBS)

  0.8μL human plasma were subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66097-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 66097-1-Ig (same clone as 66097-1-PBS)

  25μg human testis tissue was subjected to SDS PAGE followed by western blot with 66097-1-Ig (ORM1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66097-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66097-1-Ig (same clone as 66097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66097-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66097-1-Ig (same clone as 66097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66097-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66097-1-Ig (same clone as 66097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66097-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66097-1-Ig (same clone as 66097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66097-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66097-1-Ig (same clone as 66097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66097-1-Ig (ORM1/2 antibody at dilution of 1:50. This data was developed using the same antibody clone with 66097-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66097-1-Ig (same clone as 66097-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 66097-1-Ig (ORM1/2 antibody at dilution of 1:50. This data was developed using the same antibody clone with 66097-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66097-1-Ig (same clone as 66097-1-PBS)

  Immunofluorescent analysis of () fixed HepG2 cells using 66097-1-Ig (ORM1/2 antibody) at dilution of 1:25. This data was developed using the same antibody clone with 66097-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66097-1-Ig (same clone as 66097-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human ORM1/2 (66097-1-Ig, Clone:5F2H7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66097-1-PBS in a different storage buffer formulation.

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Product Information

66097-1-PBS targets ORM1/2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ORM1/2 fusion protein Ag19248
• Full Name: orosomucoid 1
• Calculated Molecular Weight: 201 aa, 24 kDa
• Observed Molecular Weight: 40-47 kDa
• GenBank Accession Number: BC026238
• Gene Symbol: ORM1
• Gene ID (NCBI): 5004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Alpha-1-acid glycoprotein 1 (AGP1), also called orosomucoid-1 (ORM1), is a glycoprotein synthesized mostly by hepatocytes and present in human plasma. ORM1 is an acute-phase reactant protein controlled by glucocorticoids, interleukin-1 and interleukin-6, and increase up to 5-50 times upon infection and/or inflammation. Anti-apoptotic effect and role as immunomodulator of ORM have been reported. ORM is an important carrier for synthetic drugs and influences their distribution and availability in the body. This antibody recognizes a band about 44 kDa in human plasma which may be due to the glycosylation of ORM1 or the dimer formation of the protein. This antibody recognizes both ORM1 and ORM2.