HRP-conjugated GST Tag Monoclonal antibody

GST Tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein, schistosoma japonicum and More (1)

Applications

WB, IP, CoIP

Conjugate

HRP

Publications

46

CloneNo.

3G12B10

Cat no : HRP-66001

Synonyms

GST, GST tag

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Validation Data Gallery

• 

  WB analysis of Recombinant protein using HRP-66001

  Recombinant protein were subjected to SDS PAGE followed by western blot with HRP-66001 (GST Tag antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:10000-1:100000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 34 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

HRP-66001 targets GST Tag in WB, IP, CoIP applications and shows reactivity with recombinant protein, schistosoma japonicum samples.

• Tested Reactivity: recombinant protein, schistosoma japonicum
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: GST Tag
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: -
• GenBank Accession Number: FN315687
• RRID: AB_2883833
• Conjugate: HRP
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

Publications

Species	Application	Title
		Nature; Allele-selective lowering of mutant HTT protein by HTT-LC3 linker compounds.; Authors - Zhaoyang Li
	WB	Cell Metab; Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export.; Authors - Ke Wang
		Cell Res; Degradation of lipid droplets by chimeric autophagy-tethering compounds.; Authors - Yuhua Fu
	CoIP	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
	WB	Nat Commun; Pharmaceutical targeting of OTUB2 sensitizes tumors to cytotoxic T cells via degradation of PD-L1; Authors - Wenfeng Ren
	WB	Nat Commun; PEBP balances apoptosis and autophagy in whitefly upon arbovirus infection.; Authors - Shifan Wang

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