HRP-conjugated GST Tag Antibody 0 Publications

Mouse Monoclonal| Catalog number: HRP-66001

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Freight/Packing

Con: 200 μg/100 μl

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Species specificity:
recombinant protein, schistosoma japonicum

Positive WB detected in:
Recombinant protein

Recommended dilution:
WB : 1:5000-1:50000

Product Information


Source:
Mouse

Purification method:
Antigen affinity purification

Isotype:
IgG2a

Storage:
PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Recombinant Protein

Full name:
GST Tag

Calculated molecular weight:
26 kDa

Observed molecular weight:
-

GenBank accession number:

Gene ID (NCBI):

Gene symbol

Synonyms
Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.


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