HRP-conjugated GST Tag Monoclonal antibody
GST Tag Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2a
Reactivity
recombinant protein, schistosoma japonicum and More (2)
Applications
WB, IP, CoIP
Conjugate
HRP
CloneNo.
3G12B10
Cat no : HRP-66001
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Recombinant protein |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:10000-1:100000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 32 publications below |
IP | See 3 publications below |
CoIP | See 1 publications below |
Product Information
HRP-66001 targets GST Tag in WB, IP, CoIP applications and shows reactivity with recombinant protein, schistosoma japonicum samples.
Tested Reactivity | recombinant protein, schistosoma japonicum |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Recombinant Protein |
Full Name | GST Tag |
Calculated Molecular Weight | 26 kDa |
Observed Molecular Weight | - |
GenBank Accession Number | FN315687 |
Gene Symbol | |
Gene ID (NCBI) | |
RRID | AB_2883833 |
Conjugate | HRP |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
Publications
Species | Application | Title |
---|---|---|
Cell Metab Di-methylation of CD147-K234 Promotes the Progression of NSCLC by Enhancing Lactate Export. | ||
Nat Commun Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence | ||
Nat Commun Pharmaceutical targeting of OTUB2 sensitizes tumors to cytotoxic T cells via degradation of PD-L1 | ||