GST Tag Monoclonal antibody, PBS Only

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  WB analysis of Recombinant protein using 66001-2-Ig (same clone as 66001-2-PBS)

  GST tagged recombinant protein (25ng/lane) was subjected to SDS-PAGE followed by western blot with anti-GST tag monoclonal antibody 66001-2-Ig at various dilutions. This data was developed using the same antibody clone with 66001-2-PBS in a different storage buffer formulation.

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  WB analysis of Recombinant protein using 66001-2-Ig (same clone as 66001-2-PBS)

  GST protein (25 ng), pGEX6P-1 vector expressed in E. coli, were subjected to SDS PAGE followed by western blot with 66001-2-Ig (GST Tag antibody, 1:200,000) incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66001-2-PBS in a different storage buffer formulation.

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  IP experiment of Recombinant protein using 66001-2-Ig (same clone as 66001-2-PBS)

  IP result of anti-GST Tag (IP:66001-2-Ig, 5ug; Detection:66001-2-Ig 1:10000) with Total E. coli lysate (with pGEX4T fused plasmid) 1360 ug. This data was developed using the same antibody clone with 66001-2-PBS in a different storage buffer formulation.

GST Tag Monoclonal Antibody for WB, IP, Indirect ELISA

Cat No. 66001-2-PBS

Clone No.3G12B10

Host / Isotype

Mouse / IgG2a

Reactivity

Recombinant protein, Schistosoma japonicum

Applications

WB, IP, Indirect ELISA

Synonyms

GST, GST Tag

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Product Information

66001-2-PBS targets GST Tag in WB, IP, Indirect ELISA applications and shows reactivity with Recombinant protein, Schistosoma japonicum samples.

• Tested Reactivity: Recombinant protein, Schistosoma japonicum
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: GST Tag
• Calculated Molecular Weight: 26 kDa
• GenBank Accession Number: FN315687
• RRID: AB_2881488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST (Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the N- or C-terminus of targeted proteins.