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Anti-Human CD107a / LAMP1 (H4A3) Mouse IgG1 Recombinant Antibody, PBS Only

CD107a / LAMP1 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

H4A3

Cat no : 65519-1-PBS

Synonyms

LAMP1, CD107a, H4A3, LAMP 1, CD107 antigen-like family member A, LAMP-1, LAMPA, LGP120, Lysosome-associated membrane glycoprotein 1, Lysosome-associated membrane protein 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using Anti-Human CD107a (H4A3) (65519-1-PBS)

    FC experiment of HeLa using 65519-1-PBS

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HeLa cells using Anti-Human CD107a (H4A3) (65519-1-PBS)

    FC experiment of HeLa using 65519-1-PBS

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of HeLa cells using Anti-Human CD107a (H4A3) (65519-1-PBS)

    FC experiment of HeLa using 65519-1-PBS

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human CD107a Mouse Recombinant Antibody (65519-1-PBS, Clone:H4A3) (left and red) or 0.2 ug Anti-Human CD107a Mouse Monoclonal Antibody (65051-1-Ig, Clone:H4A3) (center and black dashed) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) (blue) and CoraLite®647-conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L) (SA00014-8). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

65519-1-PBS targets CD107a / LAMP1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Immunogen Human adherent peripheral blood cells
Full Name lysosomal-associated membrane protein 1
GenBank Accession NumberBC006345
Gene Symbol LAMP1
Gene ID (NCBI) 3916
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Protocols

Product Specific Protocols
FC protocol for CD107a / LAMP1 antibody 65519-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols