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PCMT1 Monoclonal antibody

PCMT1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3C8G8

Cat no : 60172-1-Ig

Synonyms

PCMT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PCMT1 Monoclonal antibody (60172-1-Ig)

    WB analysis using 60172-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using PCMT1 Monoclonal antibody (60172-1-Ig)

    WB analysis of pig brain using 60172-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using PCMT1 Monoclonal antibody (60172-1-Ig)

    WB analysis of Raji using 60172-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PCMT1 Monoclonal antibody (60172-1-Ig)

    IF Staining of HeLa using 60172-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PCMT1 antibody (60172-1-Ig, Clone: 3C8G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, Raji cells, pig brain tissue, HEK-293 cells, HepG2 cells, K-562 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60172-1-Ig targets PCMT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PCMT1 fusion protein Ag6277
Full Name protein-L-isoaspartate (D-aspartate) O-methyltransferase
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25-27 kDa
GenBank Accession NumberBC008748
Gene Symbol PCMT1
Gene ID (NCBI) 5110
RRIDAB_10695773
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.

Protocols

Product Specific Protocols
WB protocol for PCMT1 antibody 60172-1-IgDownload protocol
IF protocol for PCMT1 antibody 60172-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols