PCMT1 Monoclonal antibody, PBS Only

PCMT1 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C8G8

Cat no : 60172-1-PBS

Synonyms

PCMT1

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Validation Data Gallery

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  WB analysis using 60172-1-Ig (same clone as 60172-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60172-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 60172-1-Ig (same clone as 60172-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60172-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Raji using 60172-1-Ig (same clone as 60172-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 60172-1-Ig (PCMT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60172-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60172-1-Ig (same clone as 60172-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PCMT1 antibody (60172-1-Ig, Clone: 3C8G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60172-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60172-1-PBS targets PCMT1 in WB, IF, Indirect ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PCMT1 fusion protein Ag6277
• Full Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-27 kDa
• GenBank Accession Number: BC008748
• Gene Symbol: PCMT1
• Gene ID (NCBI): 5110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.