CoraLite®594-conjugated PCMT1 Monoclonal antibody

PCMT1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C8G8

Cat no : CL594-60172

Synonyms

PCMT1

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Validation Data Gallery

• 

  IF Staining of HeLa using CL594-60172

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-60172 (PCMT1 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60172 targets PCMT1 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PCMT1 fusion protein Ag6277
• Full Name: protein-L-isoaspartate (D-aspartate) O-methyltransferase
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-27 kDa
• GenBank Accession Number: BC008748
• Gene Symbol: PCMT1
• Gene ID (NCBI): 5110
• RRID: AB_2883432
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The PCMT1 gene encodes the protein repair enzyme protein-L-isoaspartate (D-aspartate) O-methyltransferase, which is known to protect certain neural cells against Bax-induced apoptosis.