Anticorps Polyclonal de lapin anti-Endostatin

Endostatin Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, poisson-zèbre et plus (1)

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 18301-1-AP

Synonymes

COL18A1, Collagen alpha 1(XVIII) chain, collagen, type XVIII, alpha 1, Endostatin, KNO, KNO1

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Galerie de données de validation

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  WB analysis of human kidney using 18301-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 18301-1-AP (Endostatin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 18301-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18301-1-AP (Endostatin antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 18301-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 18301-1-AP (Endostatin antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of zebrafish retina using 18301-1-AP

  Immunofluorescence analysis of Collagen18a1 (aka Endostatin) polyclonal antibody (18301-1-AP, green) staining on tissue sections of the zebrafish retina at 3 days post fertilisation. Dilution 1:200. Tissue fixed overnight in 4% PFA. DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.

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  IF Staining of zebrafish retina using 18301-1-AP

  Immunofluorescence analysis of the zebrafish retina at 5 days post fertilisation. Collagen18a1 (aka Endostatin) polyclonal antibody (18301-1-AP, magenta). Dilution 1:200. Glutamine synthetase (66323-1-Ig, green) dilution 1:500. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.

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Applications testées

• Résultats positifs en WB: tissu rénal humain,
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: zebrafish retina,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

18301-1-AP cible Endostatin dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, poisson-zèbre

• Réactivité: Humain, poisson-zèbre
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: collagen, type XVIII, alpha 1
• Masse moléculaire calculée: 178 kDa
• Poids moléculaire observé: 20 kDa
• Numéro d’acquisition GenBank: BC063833
• Symbole du gène: COL18A1/Endostatin
• Identification du gène (NCBI): 80781
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from collagen alpha-1(XVIII) chain (COL18A1). It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. It potently inhibits endothelial cell proliferation and inhibits endothelial cell angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling. Endostatin is a broad spectrum angiogenesis inhibitor and may interfere with the pro-angiogenic action of growth factors such as basic fibroblast growth factor (bFGF/FGF-2) and vascular endothelial growth factor (VEGF). This antibody raised against 1693-1706 aa of human collagen alpha-1(XVIII) chain can recognize endostatin (20 kDa) and several endostatin-related fragments of higher molecular weight (PMID: 15545970; 19358707).

Publications

Species	Application	Title
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	WB	Cancer Lett; Systemic administration of mesenchymal stem cells loaded with a novel oncolytic adenovirus carrying IL-24/endostatin enhances glioma therapy.; Authors - Junhe Zhang
human	WB	FASEB J; MicroRNA-3064-5p sponged by MALAT1 suppresses angiogenesis in human hepatocellular carcinoma by targeting the FOXA1/CD24/Src pathway.; Authors - Pei Zhang
human	WB	Biotechnol Lett; PMID: 26830094; Authors - Baolong Wang
rat	WB	Neural Regen Res; miR-181b promotes angiogenesis and neurological function recovery after ischemic stroke; Authors - Li-Xia Xue

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