Anticorps Polyclonal de lapin anti-VPS33B

• Phare
• Validé par KD/KO

VPS33B Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

12

Avis (1)

N° de cat : 12195-1-AP

Synonymes

hVPS33B, VPS33B

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Galerie de données de validation

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  WB analysis of HEK-293 using 12195-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12195-1-AP

  WB result of VPS33B antibody (12195-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS33B transfected HEK-293 cells.

• 

  WB analysis of L02 using 12195-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12195-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12195-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12195-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12195-1-AP (VPS33B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12195-1-AP (VPS33B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12195-1-AP (VPS33B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 12195-1-AP (VPS33B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12195-1-AP (VPS33B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12195-1-AP (VPS33B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human spleen using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human medulloblastoma using 12195-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12195-1-AP (VPS33B antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 12195-1-AP

  Immunofluorescent analysis of MCF-7 cells, using VPS33B antibody 12195-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules L02, tissu de muscle squelettique de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cérébral humain, tissu de médulloblastome humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

12195-1-AP cible VPS33B dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: VPS33B Protéine recombinante Ag2833
• Nom complet: vacuolar protein sorting 33 homolog B (yeast)
• Masse moléculaire calculée: 617 aa, 71 kDa
• Poids moléculaire observé: 65-71 kDa
• Numéro d’acquisition GenBank: BC016445
• Symbole du gène: VPS33B
• Identification du gène (NCBI): 26276
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

Publications

Species	Application	Title
human,mouse	WB,IF,IP	Nat Genet; Mutations in VIPAR cause an arthrogryposis, renal dysfunction and cholestasis syndrome phenotype with defects in epithelial polarization.; Authors - Cullinane Andrew R AR ; KD Validated
mouse	WB	Nat Cell Biol; Circadian control of the secretory pathway maintains collagen homeostasis.; Authors - Joan Chang
human	WB,IF	Proc Natl Acad Sci U S A; Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.; Authors - Wong Dennis D
human	IF	J Cell Sci; Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.; Authors - van der Kant Rik R
human	WB	Clin Kidney J; Glomerular involvement in the arthrogryposis, renal dysfunction and cholestasis syndrome.; Authors - Amelia Holme
human	IHC, WB, CoIP, ChIP	Cancer Sci; VPS33B interacts with NESG1 to suppress cell growth and cisplatin chemoresistance in ovarian cancer.; Authors - Yingxia Ning

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

RICHA (Verified Customer) (11-03-2020)

Excellent antibody for wester and IF.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse fibroblast

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