VPS33A Polyclonal antibody

VPS33A Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 16896-1-AP

Synonyms

hVPS33A, VPS33A

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Validation Data Gallery

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  WB analysis of human brain using 16896-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16896-1-AP (VPS33A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16896-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16896-1-AP (VPS33A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 16896-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 16896-1-AP (VPS33A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16896-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16896-1-AP (VPS33A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16896-1-AP

  IP result of anti-VPS33A (IP:16896-1-AP, 4ug; Detection:16896-1-AP 1:600) with HepG2 cells lysate 1600ug.

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Tested Applications

• Positive WB detected in: human brain tissue, HepG2 cells, human cerebellum tissue, NIH/3T3 cells
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IF: See 2 publications below

Product Information

16896-1-AP targets VPS33A in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS33A fusion protein Ag10422
• Full Name: vacuolar protein sorting 33 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 596 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC016617
• Gene Symbol: VPS33A
• Gene ID (NCBI): 65082
• RRID: AB_2214916
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VPS33A is a Sec1/Munc18 family member and is a component of the homotypic fusion and protein sorting (HOPS)-tethering complex. The mammalian HOPS complex comprises six subunits (VPS11, VPS16, VPS18, VPS33A, VPS39, and VPS41) (PMID: 23901104). This complex is required for late endosome-lysosome and autophagosome-lysosome fusion (PMID: 24554770). VPS33A plays a role in vesicular transport to the lysosomal compartment.

Publications

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
mouse	WB	J Hazard Mater; VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes; Authors - Tao Wang
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB	Autophagy; Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.; Authors - Qiuhong Shen
human	IF	Cell Rep; Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes; Authors - Ariane Schleinitz
human	WB	J Cell Biol; The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes.; Authors - Rituraj Marwaha

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