VPS45 Polyclonal antibody

VPS45 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12616-1-AP

Synonyms

h VPS45, H1, H1VPS45, hlVps45, VPS45, VPS45A, VPS45B, VPS54A, VSP45, VSP45A

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 12616-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12616-1-AP (VPS45 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12616-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12616-1-AP (VPS45 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat pancreas using 12616-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 12616-1-AP (VPS45 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12616-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12616-1-AP (VPS45 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12616-1-AP

  IP result of anti-VPS45 (IP:12616-1-AP, 4ug; Detection:12616-1-AP 1:300) with mouse kidney tissue lysate 3000 ug.

---

Tested Applications

• Positive WB detected in: mouse liver tissue, rat pancreas tissue, human kidney tissue, mouse brain tissue
• Positive IP detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12616-1-AP targets VPS45 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS45 fusion protein Ag3289
• Full Name: vacuolar protein sorting 45 homolog (S. cerevisiae)
• Calculated Molecular Weight: 570 aa, 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC028382
• Gene Symbol: VPS45
• Gene ID (NCBI): 11311
• RRID: AB_2215367
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)

Publications

Species	Application	Title
human	WB	J Cell Sci; The COG complex interacts with multiple Golgi SNAREs and enhances fusogenic assembly of SNARE complexes.; Authors - Laufman Orly O