VPS11 Polyclonal antibody

• Featured Product
• KD/KO Validated

VPS11 Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), IP, ELISA, IF

Conjugate

Unconjugated

Publications

7

Cat no : 19140-1-AP

Synonyms

Vacuolar protein sorting-associated protein 11 homolog, RNF108, RING finger protein 108, PP3476, hVPS11

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Validation Data Gallery

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  WB analysis using 19140-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 19140-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 19140-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 19140-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 19140-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 19140-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19140-1-AP (VPS11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 19140-1-AP

  IP result of anti-VPS11 (IP:19140-1-AP, 4ug; Detection:19140-1-AP 1:500) with HEK-293 cells lysate 1480ug.

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  FC experiment of HepG2 using 19140-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug VPS11 Polyclonal antibody (19140-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human brain tissue, mouse pancreas tissue, mouse brain tissue, human kidney tissue, human heart tissue, HeLa cells, K-562 cells, rat brain tissue
• Positive IP detected in: HEK-293 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

19140-1-AP targets VPS11 in WB, FC (Intra), IP, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPS11 fusion protein Ag6227
• Full Name: vacuolar protein sorting 11 homolog (S. cerevisiae)
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 108-112 kDa
• GenBank Accession Number: BC065563
• Gene Symbol: VPS11
• Gene ID (NCBI): 55823
• RRID: AB_10642572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vesicle-mediated protein sorting plays an important role in segregating intracellular molecules into distinct organelles. In yeast, Vps proteins are involved in the trafficking of endocytic and biosynthetic proteins to the vacuole, which functionally resembles the lysosome of higher organisms. VPS11 is the human homolog of the yeast class C Vps11 protein, a subunit of the HOPS (homotypic fusion and protein transport) complex. Mammalian Vps11 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and membrane docking/fusion reactions of late endosomes/lysosomes.

Publications

Species	Application	Title
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB,IF	J Cell Sci; Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.; Authors - van der Kant Rik R ; KO Validated
human	WB	J Mol Biol; Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B.; Authors - Morag R Hunter
human	WB, IF	J Biol Chem; Characterization of the mammalian CORVET and HOPS complexes and their modular restructuring for endosome specificity.; Authors - Rik van der Kant
human	IF,WB	J Cell Sci; The topology of lymphotoxin β receptor accumulated upon endolysosomal dysfunction dictates the NF-κB signaling outcome.; Authors - Magdalena Banach-Orłowska ; KD Validated
human	WB,IP	Biochem J; VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.; Authors - Morag R Hunter