Anticorps Polyclonal de lapin anti-UBAP1

• Phare
• Validé par KD/KO

UBAP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 12385-1-AP

Synonymes

NAG20, UAP, UBAP, UBAP 1, UBAP1, ubiquitin associated protein 1

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Galerie de données de validation

• 

  WB analysis of human heart using 12385-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12385-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12385-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 12385-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12385-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 12385-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12385-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 12385-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12385-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 12385-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12385-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12385-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12385-1-AP

  IP result of anti-UBAP1 (IP:12385-1-AP, 4ug; Detection:12385-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human lung cancer using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12385-1-AP (UBAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12385-1-AP (UBAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 12385-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 12385-1-AP

  Immunofluorescent analysis of Hela cells, using UBAP1 antibody 12385-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, cellules A431, cellules Y79, tissu cérébral de souris, tissu de thymus de souris, tissu pulmonaire de souris, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cardiaque humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

12385-1-AP cible UBAP1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: UBAP1 Protéine recombinante Ag3055
• Nom complet: ubiquitin associated protein 1
• Masse moléculaire calculée: 502 aa, 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC020950
• Symbole du gène: UBAP1
• Identification du gène (NCBI): 51271
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

UBAP1, also named as NAG20, is a 55 kDa protein which contains 2 putative tandem UBA domains at the C terminus, a coiled-coil domain, 2 possible N-glycosylation sites, 5 PKC phosphorylation sites, 7 casein kinase II phosphorylation sites, and 3 N-myristoylation sites. UBAP1 might be a potential effective diagnosis candidate for NPC and decreased expression of UBAP1 protein is a possible point of dysfunction along the pathogenesis pathway for NPC that may contribute to malignant transformation.

Publications

Species	Application	Title
human	WB	EMBO J; A Pseudomonas aeruginosa TIR effector mediates immune evasion by targeting UBAP1 and TLR adaptors.; Authors - Paul Rc Imbert
human	WB	Curr Biol; UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.; Authors - Stefani Flavia F
human	WB	J Cell Biol; VPS37A directs ESCRT recruitment for phagophore closure.; Authors - Yoshinori Takahashi
mouse	WB,IF	Cell Rep; ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells; Authors - Danping Lu
human	WB	Cell Rep; The Dynamics of TGF-β Signaling Are Dictated by Receptor Trafficking via the ESCRT Machinery.; Authors - Daniel S J Miller
human	WB, IP	Cell Rep; Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration.; Authors - Dmitri Kharitidi

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