Anticorps Polyclonal de lapin anti-TRPC6

• Phare
• Validé par KD/KO

TRPC6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 18236-1-AP

Synonymes

TRPC 6, TRP-6, TRP6, TRP 6, Transient receptor protein 6

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Galerie de données de validation

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  WB analysis of mouse brain using 18236-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500.

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  WB analysis of mouse lung using 18236-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 18236-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 18236-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18236-1-AP (TRPC6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 18236-1-AP

  IP result of anti-TRPC6 (IP:18236-1-AP, 4ug; Detection:18236-1-AP 1:300) with mouse lung tissue lysate 3600ug.

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  IHC staining of human placenta using 18236-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 18236-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18236-1-AP (TRPC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 18236-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using TRPC6 antibody (18236-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A549 using 18236-1-AP

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human TRPC6 (18236-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cerveau de souris, cellules COLO 320, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

18236-1-AP cible TRPC6 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TRPC6 Protéine recombinante Ag12976
• Nom complet: transient receptor potential cation channel, subfamily C, member 6
• Masse moléculaire calculée: 931 aa, 106 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC093658
• Symbole du gène: TRPC6
• Identification du gène (NCBI): 7225
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Transient receptor potential canonical (TRPC) channels mediate the influx of different types of cations through the cell membrane and are involved in many functions of the organism (PMID: 28508319). The TRPC subfamily consists of seven members, TRPC1, TRPC2, TRPC3, TRPC4, TRPC5, TRPC6, TRPC7 (PMID: 32872338). TRPC6 is a Ca(2+)-permeable non-selective cation channel expressed in most human tissues. TRPC6 has been considered as potential therapeutic targets for malignant glioma treatment (PMID: 35489424).

Publications

Species	Application	Title
human	FC	Cell; Regenerative Metaplastic Clones in COPD Lung Drive Inflammation and Fibrosis.; Authors - Wei Rao
mouse	WB,IF	Biomaterials; Nongenetic optical modulation of neural stem cell proliferation and neuronal/glial differentiation.; Authors - Meitian Wang
human	IF	Proc Natl Acad Sci U S A; TRPC6 channel translocation into phagosomal membrane augments phagosomal function.; Authors - Vladimir Riazanski ; KO Validated
mouse	WB	Phytomedicine; Deciphering the pharmacological mechanisms of Rostellularia procumbens (L) Nees. Extract alleviates adriamycin-induced nephropathy in vivo and in vitro; Authors - Zhongzhu Ai
mouse	WB	Br J Pharmacol; Catalpol Alleviates Adriamycin-Induced Nephropathy by Activating the SIRT1 Signalling Pathway In Vivo and In Vitro.; Authors - Jiangnan Zhang
rat	WB	Lab Invest; Amelioration of circadian disruption and calcium-handling protein defects by choline alleviates cardiac remodeling in abdominal aorta coarctation rats.; Authors - Xi He

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