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Anticorps Polyclonal de lapin anti-TNF Alpha

TNF Alpha Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (4)

Applications

WB, ELISA, Cell treatment, Transwell Assay

Conjugaison

Non conjugué

N° de cat : 17590-1-AP

Synonymes

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNF-alpha, TNFSF2, TNFα, TNF-α, Tumor necrosis factor

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using TNF Alpha Polyclonal antibody (17590-1-AP)

    WB analysis using 17590-1-AP

    Untreated THP-1 and THP-1 (treated with 80nM PMA overnight, replaced the culture medium with 100 ng/ml LPS for 6 hours with addition of 300ng/ml Brefeldin A for the last 3 hours) cells were subjected to SDS PAGE followed by western blot with 17590-1-AP (TNF Alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using TNF Alpha Polyclonal antibody (17590-1-AP)

    WB analysis of RAW 264.7 using 17590-1-AP

    LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 17590-1-AP (TNF alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Applications testées

Résultats positifs en WBcellules THP-1, cellules RAW 264.7 traitées au LPS

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

17590-1-AP cible TNF Alpha dans les applications de WB, ELISA, Cell treatment, Transwell Assay et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, bovin, Humain, Lapin, souris, fish
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène TNF Alpha Protéine recombinante Ag11433
Nom complet tumor necrosis factor (TNF superfamily, member 2)
Masse moléculaire calculée 233 aa, 26 kDa
Poids moléculaire observé 26 kDa
Numéro d’acquisition GenBankBC028148
Symbole du gène TNF alpha
Identification du gène (NCBI) 7124
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, ins resistance, and cancer.

Protocole

Product Specific Protocols
WB protocol for TNF Alpha antibody 17590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Brain Behav Immun

HMGB1 mediates depressive behavior induced by chronic stress through activating the kynurenine pathway.

Authors - Bo Wang
View Article
mouse

Bioact Mater

A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds

Authors - Shunlai Shang
View Article
mouseWB

ACS Cent Sci

Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury

Authors - Juan Zhang
View Article
mouseWB

Adv Sci (Weinh)

3D Printing of a Vascularized Mini-Liver Based on the Size-Dependent Functional Enhancements of Cell Spheroids for Rescue of Liver Failure

Authors - Jiabin Zhang
View Article
WB

Small

M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation

Authors - Jie Sun
View Article
ratWB

Gut Microbes

S-amlodipine induces liver inflammation and dysfunction through the alteration of intestinal microbiome in a rat model

Authors - Xinxin Liu
View Article

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Margarita (Verified Customer) (11-22-2023)

Prostate Cancer cell line, overnight incubation, Mouse

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: TRAMPC C2
TNF Alpha Antibody Immunofluorescence validation (1:100 dilution) in TRAMPC C2 (Cat no:17590-1-AP)
FH

Isha (Verified Customer) (09-20-2021)

signal was very good. My sample was renal tissue lysate after ischemia reperfusion

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:800
  • Cell Tissue Type: Kidney