IL-1RA Polyclonal antibody

IL-1RA Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10844-1-AP

Synonyms

Anakinra, ICIL 1RA, IL 1ra, IL 1ra3, IL 1RN, IL1 inhibitor, IL1F3, IL1RA, IL-1RA, IL1RN, IRAP



Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inhuman oesophagus tissue, human colon tissue, human colon cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10844-1-AP targets IL-1RA in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-1RA fusion protein Ag1277
Full Name interleukin 1 receptor antagonist
Calculated Molecular Weight 20 kDa
Observed Molecular Weight18-20 kDa
GenBank Accession NumberBC009745
Gene Symbol IL1RN
Gene ID (NCBI) 3557
RRIDAB_2125559
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IL-1RA antibody 10844-1-APDownload protocol
IHC protocol for IL-1RA antibody 10844-1-APDownload protocol
IF protocol for IL-1RA antibody 10844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Transl Oncol

Inhibitory effect and mechanism of mesenchymal stem cells on melanoma cells.

Authors - J Zhang
humanWB

NeuroImmune Pharm Ther

Resveratrol and its analogs suppress HIV replication, oxidative stress, and inflammation in macrophages

Authors - Santosh Kumar
mouseIF

Front Immunol

The influence of angiopoietin-like protein 3 on macrophages polarization and its effect on the podocyte EMT in diabetic nephropathy

Authors - Yanli Ma