Anticorps Polyclonal de lapin anti-TET2

• Phare
• Validé par KD/KO

TET2 Polyclonal Antibody for WB, IP, IHC, ELISA, IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, ELISA

Conjugaison

Non conjugué

Publications

63

Avis (2)

N° de cat : 21207-1-AP

Synonymes

Nbla00191, Methylcytosine dioxygenase TET2, KIAA1546, EC:1.14.11.80

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Galerie de données de validation

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  WB analysis using 21207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21207-1-AP (TET2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 21207-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21207-1-AP (TET2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21207-1-AP

  IP result of anti-TET2 (IP:21207-1-AP, 3ug; Detection:21207-1-AP 1:500) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 21207-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21207-1-AP (TET2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21207-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21207-1-AP (TET2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Neuro-2a using 21207-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using 21207-1-AP (TET2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 21207-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TET2 (21207-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HL-60, cellules K-562
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Neuro-2a,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 13 publications below
• WB: See 53 publications below
• IHC: See 10 publications below
• IF: See 13 publications below
• CoIP: See 3 publications below
• ChIP: See 4 publications below

Informations sur le produit

21207-1-AP cible TET2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, CoIP, chIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Chèvre, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: TET2 Protéine recombinante Ag15583
• Nom complet: tet oncogene family member 2
• Masse moléculaire calculée: 2002 aa, 224 kDa
• Poids moléculaire observé: 130 kDa, 250 kDa
• Numéro d’acquisition GenBank: BC150180
• Symbole du gène: TET2
• Identification du gène (NCBI): 54790
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TET2 is an enzyme which converts 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) in DNA and is important for normal myelopoiesis. TET2 mutations are frequently observed in myeloid malignancies, including myeloproliferative neoplasms. TET2 is broadly expressed in a variety of tissues. In healthy tissues, TET2 expression was shown to be elevated in hematopoietic cells with highest expression in granulocytes. Three isoforms of TET2 exists due to the alternative splicing. 220-250 kDa bands represent the full-length isoform; 130-133 kDa bands represent the nuclear isoforms of TET2. (PMID: 24615693) This antibody recognizes all three isoforms of TET2.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); m6A-Modified circTET2 Interacting with HNRNPC Regulates Fatty Acid Oxidation to Promote the Proliferation of Chronic Lymphocytic Leukemia; Authors - Zijuan Wu
human	WB	Sci Adv; The chromosomal protein SMCHD1 regulates DNA methylation and the 2c-like state of embryonic stem cells by antagonizing TET proteins.; Authors - Zhijun Huang
mouse	CoIP	Sci Adv; Reprogramming of DNA methylation at NEUROD2-bound sequences during cortical neuron differentiation.; Authors - Maria A Hahn
mouse	WB	Nat Commun; Redirected nuclear glutamate dehydrogenase supplies Tet3 with α-ketoglutarate in neurons.; Authors - Franziska R Traube
mouse	WB	J Exp Med; TGF-β signaling controls Foxp3 methylation and T reg cell differentiation by modulating Uhrf1 activity.; Authors - Xiang Sun
mouse	WB	JACC Basic Transl Sci; Hypermethylation of the Micro-RNA 145 Promoter Is the Key Regulator for NLRP3 Inflammasome-Induced Activation and Plaque Formation.; Authors - Wei Zhong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Melanie (Verified Customer) (11-19-2019)

IHC of 5um thin skin sections was performed. A positive staining was observed in the skin epithelial layer as predicted by the human protein atlas. Citrate buffer pH6 was used for antigen retrieval

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Skin

TET2 Antibody Immunohistochemistry, validation (1:400 dilution) in Skin (Cat no:21207-1-AP)

FH

Sid (Verified Customer) (04-12-2019)

Product performed well in western blot

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: K562

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