IDH2 Polyclonal antibody

• Featured Product
• KD/KO Validated

IDH2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

49

Reviews (1)

Cat no : 15932-1-AP

Synonyms

ICD M, IDH, IDH2, IDHM, IDP, IDPM, mNADP IDH, NADP(+) specific ICDH, Oxalosuccinate decarboxylase

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Validation Data Gallery

• 

  WB analysis using 15932-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15932-1-AP

  WB result of IDH2 antibody (15932-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDH2 transfected HEK-293 cells.

• 

  WB analysis of mouse liver using 15932-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15932-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15932-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15932-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15932-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 15932-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 15932-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15932-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15932-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15932-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15932-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15932-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 15932-1-AP

  IP result of anti-IDH2 (IP:15932-1-AP, 3ug; Detection:15932-1-AP 1:1000) with mouse brain tissue lysate 6000ug.

• 

  IHC staining of human colon cancer using 15932-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15932-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15932-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15932-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15932-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15932-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15932-1-AP (IDH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, HeLa cells, mouse heart tissue, mouse skeletal muscle tissue, mouse liver tissue, rat liver tissue, NIH/3T3 cells, SH-SY5Y cells, mouse brain tissue, HepG2 cells, K-562 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human colon cancer tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 44 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

15932-1-AP targets IDH2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IDH2 fusion protein Ag8779
• Full Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial
• Calculated Molecular Weight: 452 aa, 51 kDa
• Observed Molecular Weight: 41-47 kDa
• GenBank Accession Number: BC009244
• Gene Symbol: IDH2
• Gene ID (NCBI): 3418
• RRID: AB_2264612
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.

Publications

Species	Application	Title
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma
mouse	WB	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan
pig	WB	Sci Total Environ; Protein lysine acetylation played an important role in NH3-induced AEC2 damage and pulmonary fibrosis in piglets; Authors - Xiaotong Wang
human	WB,IHC	Mol Syst Biol; STAT3-dependent analysis reveals PDK4 as independent predictor of recurrence in prostate cancer.; Authors - Monika Oberhuber
human	WB	Cancer Res; SIRT3-Mediated Dimerization of IDH2 Directs Cancer Cell Metabolism and Tumor Growth.; Authors - Xianghui Zou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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