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  • KD/KO Validated

EZH2 Polyclonal antibody

EZH2 Polyclonal Antibody for WB, IP, IF, FC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 21800-1-AP


Enhancer of zeste homolog 2, ENX 1, ENX-1, EZH1, EZH2, KMT6, Lysine N methyltransferase 6

Tested Applications

Positive WB detected inDU 145 cells, HEK-293 cells, mouse kidney tissue, NIH/3T3 cells, Raji cells, Jurkat cells, A431 cells, HepG2 cells, rat kidney tissue, C6 cells
Positive IP detected inHepG2 cells
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21800-1-AP targets EZH2 in WB, IP, IF, FC, RIP, CoIP, CHIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, sheep, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EZH2 fusion protein Ag16789
Full Name enhancer of zeste homolog 2 (Drosophila)
Calculated Molecular Weight 751 aa, 86 kDa
Observed Molecular Weight 90-102 kDa
GenBank Accession NumberBC010858
Gene Symbol EZH2
Gene ID (NCBI) 2146
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)


Product Specific Protocols
WB protocol for EZH2 antibody 21800-1-APDownload protocol
IF protocol for EZH2 antibody 21800-1-APDownload protocol
IP protocol for EZH2 antibody 21800-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Circ Res

VE-Cadherin-Mediated Epigenetic Regulation of Endothelial Gene Expression.

Authors - Marco F Morini

Nat Struct Mol Biol

lnc-β-Catm elicits EZH2-dependent β-catenin stabilization and sustains liver CSC self-renewal.

Authors - Pingping Zhu

Cancer Res

HBXIP and LSD1 Scaffolded by lncRNA Hotair Mediate Transcriptional Activation by c-Myc.

Authors - Yinghui Li


Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI

Authors - Bojun Li
  • KD Validated

Nat Commun

CDYL suppresses epileptogenesis in mice through repression of axonal Nav1.6 sodium channel expression.

Authors - Yongqing Liu
  • KD Validated

Clin Cancer Res

Single-Cell Analyses Reveal Mechanisms of Cancer Stem Cell Maintenance and Epithelial-Mesenchymal Transition in Recurrent Bladder Cancer.

Authors - Huanjun Wang
  • KD Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Nikhil (Verified Customer) (10-16-2019)

very nice

  • Applications: Western Blot,
  • Cell Tissue Type: Colon cancer cells
EZH2 Antibody Western Blot, validation ( dilution) in Colon cancer cells (Cat no:21800-1-AP)