Anticorps Polyclonal de lapin anti-SREBF2

• Phare
• Validé par KD/KO

SREBF2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, chIP, ELISA

Conjugaison

Non conjugué

Publications

35

N° de cat : 14508-1-AP

Synonymes

bHLHd2, SREBF2, SREBP 2, SREBP2

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Galerie de données de validation

• 

  WB analysis of Jurkat using 14508-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14508-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14508-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14508-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 14508-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 14508-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 14508-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 14508-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 14508-1-AP

  IP result of anti-SREBF2 (IP:14508-1-AP, 3ug; Detection:14508-1-AP 1:500) with HeLa cells lysate 3000ug.

• 

  IHC staining of mouse brain using 14508-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14508-1-AP (SREBF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14508-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14508-1-AP (SREBF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14508-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14508-1-AP (SREBF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14508-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14508-1-AP (SREBF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 14508-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14508-1-AP (SREBF2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 14508-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14508-1-AP (SREBF2 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa, cellules HepG2, tissu hépatique de rat, tissu rénal de rat, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 33 publications below
• IHC: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

14508-1-AP cible SREBF2 dans les applications de WB, IP, IHC, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SREBF2 Protéine recombinante Ag5967
• Nom complet: sterol regulatory element binding transcription factor 2
• Masse moléculaire calculée: 124 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC056158
• Symbole du gène: SREBF2
• Identification du gène (NCBI): 6721
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues.

Publications

Species	Application	Title
mouse	WB	Nature; The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1.; Authors - Jinbo Han
mouse	WB	Nature; TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis.; Authors - Joanne Hsieh
mouse	WB	Environ Pollut; Prenatal exposure to diesel exhaust PM2.5 programmed non-alcoholic fatty liver disease differently in adult male offspring of mice fed normal chow and a high-fat diet.; Authors - Xiaoke Wang
human, mouse	WB	Arterioscler Thromb Vasc Biol; RP5-833A20.1/miR-382-5p/NFIA-Dependent Signal Transduction Pathway Contributes to the Regulation of Cholesterol Homeostasis and Inflammatory Reaction.; Authors - Yan-Wei Hu
mouse, rat	WB	Arch Toxicol; Protective effects of dioscin against alcohol-induced liver injury.; Authors - Xu Tingting T
mouse	IHC	J Lipid Res; Intracellular hepatitis B virus increases hepatic cholesterol deposition in alcoholic fatty liver via hepatitis B core protein.; Authors - Ya-Qi Wang

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