INSIG1 Polyclonal antibody

• Featured Product
• KD/KO Validated

INSIG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 22115-1-AP

Synonyms

CL 6, INSIG 1, INSIG1, insulin induced gene 1, Insulin induced gene 1 protein

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Validation Data Gallery

• 

  WB analysis of A549 using 22115-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22115-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 22115-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 22115-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 22115-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22115-1-AP (INSIG1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 22115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 22115-1-AP (INSIG1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells, SGC-7901 cells, MCF-7 cells
• Positive IHC detected in: human liver tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

The immunogen of 22115-1-AP is INSIG1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INSIG1 fusion protein Ag17420
• Full Name: insulin induced gene 1
• Calculated Molecular Weight: 277 aa, 30 kDa
• Observed Molecular Weight: 28-31 kDa, 66-70 kDa
• GenBank Accession Number: BC001880
• Gene Symbol: INSIG1
• Gene ID (NCBI): 3638
• RRID: AB_11183051
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-induced genes (INSIGs), which consist of two isoforms (INSIG1 and INSIG2), are representative endoplasmic reticulum (ER)-resident oxysterol-binding proteins (OSBPs). INSIG1 is part of negative regulatory feedback, acting as a brake on SREBP transcriptional function (PMID: 23919961). INSIG1 also has a secondary function mediating sterolaccelerated proteolytic degradation of HMG CoA reductase, thus impacting the mevalonate pathway. Suppression of INSIG1 function promotes hepatic lipid remodelling and restrains NASH (non-alcoholic steatohepatitis) progression(PMID: 33722690).

Publications

Species	Application	Title
human	WB,IF	Nature; The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.; Authors - Daqian Xu ; KD Validated
	WB	Transl Psychiatry; A potential mechanism underlying atypical antipsychotics-induced lipid disturbances.; Authors - H L Cai
	WB	Eur Neuropsychopharmacol; Vitamin D deficiency exacerbates atypical antipsychotic-induced metabolic side effects in rats: Involvement of the INSIG/SREBP pathway.; Authors - Ruili Dang
	WB	Mol Nutr Food Res; (-)-Epicatechin regulates blood lipids and attenuates hepatic steatosis in rats fed high-fat diet(†).; Authors - Hui Cheng
	WB,IHC	J Physiol Biochem; Renal tubule ectopic lipid deposition in diabetic kidney disease rat model and in vitro mechanism of leptin intervention.; Authors - Shasha Liu
human	IF	JCI Insight; Hypomorphic ASGR1 modulates lipid homeostasis via INSIG1-mediated SREBP signaling suppression.; Authors - Yingying Xu