Validation Data Gallery
|Positive WB detected in||HeLa cells, Jurkat cells, human brain tissue, Raji cells, mouse brain tissue, HL-60 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse brain tissue, human brain tissue, mouse liver tissue, human pancreas tissue, human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
10785-1-AP targets LDLR in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, Chicken, Hamster, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LDLR fusion protein Ag1236|
|Full Name||low density lipoprotein receptor|
|Calculated molecular weight||95 kDa|
|Observed molecular weight||100-160 kDa|
|GenBank accession number||BC014514|
|Gene ID (NCBI)||3949|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.
|Product Specific Protocols|
|WB protocol for LDLR antibody 10785-1-AP||Download protocol|
|IHC protocol for LDLR antibody 10785-1-AP||Download protocol|
|IF protocol for LDLR antibody 10785-1-AP||Download protocol|
|IP protocol for LDLR antibody 10785-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.
Identification and evaluation of a lipid-lowering small compound in preclinical models and in a Phase I trial.
J Clin Invest
Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation.
A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry.
Adv Healthc Mater
Engineered Red Blood Cell Membrane-Coating Salidroside/Indocyanine Green Nanovesicles for High-Efficiency Hypoxic Targeting Phototherapy of Triple-Negative Breast Cancer.
Acetaldehyde Dehydrogenase 2 regulates HMG-CoA reductase stability and cholesterol synthesis in the liver.