Anticorps Polyclonal de lapin anti-SQLE

• Phare
• Validé par KD/KO

SQLE Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

80

N° de cat : 12544-1-AP

Synonymes

ERG1, SE, SM, SQLE, squalene epoxidase, Squalene monooxygenase

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Galerie de données de validation

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  WB analysis using 12544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12544-1-AP (SQLE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12544-1-AP

  IP result of anti-SQLE (IP:12544-1-AP, 4ug; Detection:12544-1-AP 1:500) with HepG2 cells lysate 2240 ug.

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  IHC staining of human prostate cancer using 12544-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12544-1-AP (SQLE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12544-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12544-1-AP (SQLE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12544-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SQLE antibody (12544-1-AP) at dilution of 1:40 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of PC-3 using 12544-1-AP

  Immunofluorescent analysis of PC-3 cells using 12544-1-AP (SQLE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HepG2
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 15 publications below
• WB: See 72 publications below
• IHC: See 15 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

12544-1-AP cible SQLE dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SQLE Protéine recombinante Ag3266
• Nom complet: squalene epoxidase
• Masse moléculaire calculée: 574 aa, 64 kDa
• Poids moléculaire observé: 50-64 kDa
• Numéro d’acquisition GenBank: BC017033
• Symbole du gène: SQLE
• Identification du gène (NCBI): 6713
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)

Publications

Species	Application	Title
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
hamster	WB	Cell Metab; Cholesterol-dependent degradation of squalene monooxygenase, a control point in cholesterol synthesis beyond HMG-CoA reductase.; Authors - Gill Saloni S
mouse	WB	Cell Metab; PMID: 21109190; Authors - Ryo Suzuki
mouse	WB	PLoS Biol; Reduction of the cholesterol sensor SCAP in the brains of mice causes impaired synaptic transmission and altered cognitive function.; Authors - Suzuki Ryo R
human	WB,IHC	Nat Commun; MiR-205-driven downregulation of cholesterol biosynthesis through SQLE-inhibition identifies therapeutic vulnerability in aggressive prostate cancer.; Authors - C Kalogirou
human	WB	Redox Biol; Lipophagy-mediated cholesterol synthesis inhibition is required for the survival of hepatocellular carcinoma under glutamine deprivation; Authors - Youzi Kong

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