NFYA Polyclonal antibody

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  WB analysis of HEK-293 using 12981-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 12981-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12981-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12981-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12981-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12981-1-AP (NFYA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 12981-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12981-1-AP (NFYA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12981-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12981-1-AP (NFYA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12981-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12981-1-AP (NFYA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12981-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12981-1-AP (NFYA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12981-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NFYA antibody (12981-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

NFYA Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12981-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NF-YA, NF YA, HAP2, CBF B, CBF A

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HT-1080 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human colon cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

12981-1-AP targets NFYA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFYA fusion protein Ag3604
• Full Name: nuclear transcription factor Y, alpha
• Calculated Molecular Weight: 318aa,34 kDa; 347aa,39 kDa
• Observed Molecular Weight: 40 kDa, 46 kDa
• GenBank Accession Number: BC039244
• Gene Symbol: NFYA
• Gene ID (NCBI): 4800
• RRID: AB_10644128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23511
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFYA, also named as Nuclear transcription factor Y subunit alpha, is a 347 amino acid protein, which contains 1 NFYA/HAP2-type DNA-binding domain and belongs to the NFYA/HAP2 subunit family. NFYA exists the two 41- to 45-kDa isoforms of NF-YA ( 11287613 ). NFYA is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human,mouse	WB	J Virol; Minute Virus of Mice (MVM) inhibits transcription of the cyclin B1 gene during infection.; Authors - Matthew S Fuller
human	WB	Int J Oncol; Gene regulatory network construction identified NFYA as a diffuse subtype-specific prognostic factor in gastric cancer.; Authors - Bin Cao ; KD Validated
rat	WB	Cell Death Dis; PRMT5 critically mediates TMAO-induced inflammatory response in vascular smooth muscle cells.; Authors - He Liu ; KD Validated