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  • KD/KO Validated

NFYA Polyclonal antibody

NFYA Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12981-1-AP

Synonyms

CBF A, CBF B, HAP2, NF YA, NFYA



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HT-1080 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12981-1-AP targets NFYA in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NFYA fusion protein Ag3604
Full Name nuclear transcription factor Y, alpha
Calculated molecular weight318aa,34 kDa; 347aa,39 kDa
Observed molecular weight 40 kDa, 46 kDa
GenBank accession numberBC039244
Gene symbol NFYA
Gene ID (NCBI) 4800
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFYA, also named as Nuclear transcription factor Y subunit alpha, is a 347 amino acid protein, which contains 1 NFYA/HAP2-type DNA-binding domain and belongs to the NFYA/HAP2 subunit family. NFYA exists the two 41- to 45-kDa isoforms of NF-YA ( 11287613 ). NFYA is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1

Protocols

Product Specific Protocols
WB protocol for NFYA antibody 12981-1-APDownload protocol
IHC protocol for NFYA antibody 12981-1-APDownload protocol
IF protocol for NFYA antibody 12981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
human,mouseWB

J Virol

Minute Virus of Mice (MVM) inhibits transcription of the cyclin B1 gene during infection.

Authors - Matthew S Fuller
humanWB

Int J Oncol

Gene regulatory network construction identified NFYA as a diffuse subtype-specific prognostic factor in gastric cancer.

Authors - Bin Cao
  • KD Validated
ratWB

Cell Death Dis

PRMT5 critically mediates TMAO-induced inflammatory response in vascular smooth muscle cells.

Authors - He Liu
  • KD Validated