Validation Data Gallery
|Positive WB detected in||HEK-293 cells, HeLa cells, HT-1080 cells, Jurkat cells, K-562 cells|
|Positive IHC detected in||human colon cancer tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12981-1-AP targets NFYA in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||NFYA fusion protein Ag3604|
|Full Name||nuclear transcription factor Y, alpha|
|Calculated molecular weight||318aa,34 kDa; 347aa,39 kDa|
|Observed molecular weight||40 kDa, 46 kDa|
|GenBank accession number||BC039244|
|Gene ID (NCBI)||4800|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
NFYA, also named as Nuclear transcription factor Y subunit alpha, is a 347 amino acid protein, which contains 1 NFYA/HAP2-type DNA-binding domain and belongs to the NFYA/HAP2 subunit family. NFYA exists the two 41- to 45-kDa isoforms of NF-YA ( 11287613 ). NFYA is a component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1
Divergent transcriptional regulation of astrocyte reactivity across disorders.
Minute Virus of Mice (MVM) inhibits transcription of the cyclin B1 gene during infection.
Int J Oncol
Gene regulatory network construction identified NFYA as a diffuse subtype-specific prognostic factor in gastric cancer.
Cell Death Dis
PRMT5 critically mediates TMAO-induced inflammatory response in vascular smooth muscle cells.