RAP2B Polyclonal antibody

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  WB analysis using 16266-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16266-1-AP (RAP2B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16266-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16266-1-AP (RAP2B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16266-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16266-1-AP (RAP2B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 16266-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 16266-1-AP (RAP2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 16266-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16266-1-AP (RAP2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RAP2B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16266-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Ras-related protein Rap-2b, Ras related protein Rap 2b, EC:3.6.5.2

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Tested Applications

• Positive WB detected in: mouse brain tissue, MCF-7 cells, rat brain tissue, HEK-293 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16266-1-AP targets RAP2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RAP2B fusion protein Ag9331
• Full Name: RAP2B, member of RAS oncogene family
• Calculated Molecular Weight: 183 aa, 21 kDa
• Observed Molecular Weight: 19-22 kDa
• GenBank Accession Number: BC012362
• Gene Symbol: RAP2B
• Gene ID (NCBI): 5912
• RRID: AB_2177311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61225
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAP2B belongs to a family of RAS-related genes. It share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. Rap2B is a platelet protein that is activated by thrombin and involved in platelet activation. RAP2B may be a novel candidate oncogene that plays important roles in carcinogenesis through activation of NF-kappaB pathway.

Publications

Species	Application	Title
mouse	WB	J Biol Chem; DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells.; Authors - Li Yi Y