NFYC Polyclonal antibody

NFYC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10129-2-AP

Synonyms

CBF C, CBFC, H1TF2A, HAP5, HSM, NF YC, NFYC, Transactivator HSM 1/2

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Validation Data Gallery

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  WB analysis of K-562 using 10129-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10129-2-AP (NFYC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10129-2-AP targets NFYC in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NFYC fusion protein Ag0140
• Full Name: nuclear transcription factor Y, gamma
• Calculated Molecular Weight: 37 kDa, 50 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC005003
• Gene Symbol: NFYC
• Gene ID (NCBI): 4802
• RRID: AB_2152244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NFYC, also named as Nuclear transcription factor Y subunit gamma or Transactivator HSM-1/2, is a 458 amino acid protein, which belongs to the NFYC/HAP5 subunit family. NFYC localizes in the nucleus and stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. SP1, NFYC and FOXO3, FOXO4 transcription factors are involved in the regulation of STK11 transcription.

Publications

Species	Application	Title
mouse	WB	Cell Res; Pancreas-specific Pten deficiency causes partial resistance to diabetes and elevated hepatic AKT signaling.; Authors - Tong Zan Z