Anticorps Polyclonal de lapin anti-SPRR3

• Phare
• Validé par KD/KO

SPRR3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 11742-1-AP

Synonymes

22 kDa pancornulin, Cornifin beta, Esophagin, small proline rich protein 3, SPRC, SPRR3

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Galerie de données de validation

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  WB analysis of HeLa using 11742-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11742-1-AP (SPRR3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11742-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11742-1-AP (SPRR3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 11742-1-AP

  IP result of anti-SPRR3 (IP:11742-1-AP, 3ug; Detection:11742-1-AP 1:500) with COLO 320 cells lysate 1720ug.

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  IHC staining of human colon cancer using 11742-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon cancer using 11742-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 11742-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human breast cancer using 11742-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11742-1-AP (SPRR3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 11742-1-AP

  Immunofluorescent analysis of HeLa cells using 11742-1-AP (SPRR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu hépatique humain
• Résultats positifs en IP: cellules COLO 320
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

11742-1-AP cible SPRR3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SPRR3 Protéine recombinante Ag2337
• Nom complet: small proline-rich protein 3
• Masse moléculaire calculée: 19 kDa
• Poids moléculaire observé: 22-25 kDa
• Numéro d’acquisition GenBank: BC017802
• Symbole du gène: SPRR3
• Identification du gène (NCBI): 6707
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SPRR3, also named as Esophagin, Cornifin beta and SPRC, belongs to the cornifin (SPRR) family. It is cross-linked envelope protein of keratinocytes. Increased expression of SPRR3 appears to be involved in colorectal tumorigenesis. SPRR3 is a marker for terminal squamous cell differentiation.a potential novel therapeutic target for less advanced stages of Breast Cancer.

Publications

Species	Application	Title
human,mouse,rat	WB,IP	FASEB J; Small proline-rich repeat 3 is a novel coordinator of PDGFRβ and integrin β1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts.; Authors - Sarika Saraswati ; KO Validated
human	WB,IHC	Mol Med; Upregulation of SPRR3 promotes colorectal tumorigenesis.; Authors - Cho Dong-Hyung DH ; KD Validated
human	WB,IHC	Breast Cancer Res Treat; Expression of SPRR3 is associated with tumor cell proliferation in less advanced stages of breast cancer.; Authors - Kim Jin Cheon JC
human	WB	Arch Biochem Biophys; Investigation of mouse amniotic fluid for stimulating ability of keratinocyte differentiation depending on the fetal stage.; Authors - Miki Kuribayashi
human	WB	Onco Targets Ther; Dysregulation of SPRR3/miR-876-3p Axis Contributes to Tumorigenesis in Non-Small-Cell Lung Cancer.; Authors - Qin Li
human	WB	bioRxiv; Discovery of novel microRNA regulators of ribosome biogenesis; Authors - Carson J Bryant ; KD Validated

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