TGM1 Polyclonal antibody

TGM1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12912-3-AP

Synonyms

Epidermal TGase, ICR2, KTG, LI, LI1, TG(K), TGASE, TGase 1, TGase K, TGK, TGM1, Transglutaminase 1, Transglutaminase K



Tested Applications

Positive WB detected inhuman saliva, mouse kidney tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman cervical cancer tissue, human skin tissue, human cervix tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

12912-3-AP targets TGM1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TGM1 fusion protein Ag3905
Full Name transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase)
Calculated molecular weight 817 aa, 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC034699
Gene symbol TGM1
Gene ID (NCBI) 7051
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transglutaminase 1 is an epidermal enzyme that is expressed during the terminal differentiation of keratinized squamous epithelium to form cornified cell envelopes in differentiated keratinocytes. Mutation of TGM1 has been linked to various epidermal diseases. This antibody recognizes the endogenous 90 kDa TGM1 proteins.

Protocols

Product Specific Protocols
WB protocol for TGM1 antibody 12912-3-APDownload protocol
IHC protocol for TGM1 antibody 12912-3-APDownload protocol
IP protocol for TGM1 antibody 12912-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
humanWB

J Invest Dermatol

Investigation of Omega-3 Polyunsaturated Fatty Acid Biological Activity in a Tissue-Engineered Skin Model Involving Psoriatic Cells.

Authors - Mélissa Simard
humanWB

J Invest Dermatol

Cathelicidin LL-37 Activates Human Keratinocyte Autophagy through the P2X₇, Mechanistic Target of Rapamycin, and MAPK Pathways

Authors - Risa Ikutama
humanIF

Int J Mol Sci

Docosahexaenoic Acid Modulates Paracellular Absorption of Testosterone and Claudin-1 Expression in a Tissue-Engineered Skin Model.

Authors - Andréa Tremblay
humanWB

Front Endocrinol (Lausanne)

c-Myc Upregulated by High Glucose Inhibits HaCaT Differentiation by S100A6 Transcriptional Activation.

Authors - Jie Zhang
humanWB

Skin Pharmacol Physiol

Effect of Lactic Fermentation Products on Human Epidermal Cell Differentiation, Ceramide Content, and Amino Acid Production.

Authors - Moe Otsuka