TGM1 Polyclonal antibody

TGM1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

33

Cat no : 12912-3-AP

Synonyms

LI1, LI, KTG, ICR2, Epidermal TGase

---

Validation Data Gallery

• 

  WB analysis using 12912-3-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12912-3-AP (TGM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12912-3-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12912-3-AP (TGM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12912-3-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12912-3-AP (TGM1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12912-3-AP

  IP result of anti-TGM1 (IP:12912-3-AP, 4ug; Detection:12912-3-AP 1:1000) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human cervix using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human bowen disease using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12912-3-AP (TGM1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12912-3-AP (TGM1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12912-3-AP (TGM1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12912-3-AP (TGM1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human cervical cancer using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12912-3-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12912-3-AP (TGM1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: human saliva, mouse kidney tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human cervical cancer tissue, human bowen disease, human cervix tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IHC: See 5 publications below
• IF: See 11 publications below
• ELISA: See 1 publications below

Product Information

12912-3-AP targets TGM1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TGM1 fusion protein Ag3905
• Full Name: transglutaminase 1 (K polypeptide epidermal type I, protein-glutamine-gamma-glutamyltransferase)
• Calculated Molecular Weight: 817 aa, 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC034699
• Gene Symbol: TGM1
• Gene ID (NCBI): 7051
• RRID: AB_2271748
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transglutaminase 1 is an epidermal enzyme that is expressed during the terminal differentiation of keratinized squamous epithelium to form cornified cell envelopes in differentiated keratinocytes. Mutation of TGM1 has been linked to various epidermal diseases. This antibody recognizes the endogenous 90 kDa TGM1 proteins.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	J Control Release; Superior COL7A1 and TGM1 gene expression in difficult-to-transfect skin cell mediated by highly branched poly(β-amino esters) through stepwise fractionation; Authors - Chaolan Pan
human	WB	J Invest Dermatol; Investigation of Omega-3 Polyunsaturated Fatty Acid Biological Activity in a Tissue-Engineered Skin Model Involving Psoriatic Cells.; Authors - Mélissa Simard
human	WB	J Invest Dermatol; Cathelicidin LL-37 Activates Human Keratinocyte Autophagy through the P2X₇, Mechanistic Target of Rapamycin, and MAPK Pathways; Authors - Risa Ikutama
human	IF	Int J Mol Sci; Docosahexaenoic Acid Modulates Paracellular Absorption of Testosterone and Claudin-1 Expression in a Tissue-Engineered Skin Model.; Authors - Andréa Tremblay
mouse	WB,ELISA	Cells; Increased Osteoclastogenesis in Absence of TG2 Is Reversed by Transglutaminase Inhibition-Evidence for the Role for TG1 in Osteoclast Formation; Authors - Sahar Ebrahimi Samani