Involucrin Polyclonal antibody

Involucrin Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

19

Reviews (1)

Cat no : 55328-1-AP

Synonyms

involucrin, IVL

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Validation Data Gallery

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  WB analysis of Raji using 55328-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 55328-1-AP (IVL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 55328-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55328-1-AP (IVL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin cancer using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human oesophagus using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 55328-1-AP (Involucrin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 55328-1-AP (Involucrin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thymus using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skin cancer using 55328-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 55328-1-AP (Involucrin antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of mouse skin using 55328-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using 55328-1-AP (Involucrin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Raji cells, NIH/3T3 cells
• Positive IHC detected in: human skin cancer tissue, human thymus tissue, human oesophagus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 6 publications below

Product Information

55328-1-AP targets Involucrin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: involucrin
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 80-90 kDa, 56-70 kDa
• GenBank Accession Number: NM_005547
• Gene Symbol: Involucrin
• Gene ID (NCBI): 3713
• RRID: AB_11182270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502)

Publications

Species	Application	Title
human	IF	Sci Rep; Loss-of-function Mutation in PMVK Causes Autosomal Dominant Disseminated Superficial Porokeratosis.; Authors - Jiuxiang Wang
mouse	WB	J Ethnopharmacol; The Water Extracts from the Oil Cakes of Prinsepia utilis Repair the Epidermal Barrier via Up-Regulating Corneocyte Envelope-Proteins, Lipid Synthases, and Tight Junction Proteins; Authors - Ying Tu
human	WB	J Eur Acad Dermatol Venereol; Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.; Authors - T Yuan
human	WB	Oncotarget; Transcriptional suppression of microRNA-27a contributes to laryngeal cancer differentiation via GSK-3β-involved Wnt/β-catenin pathway.; Authors - Sheng Chen
human	WB	Front Oncol; Cystatin-B Negatively Regulates the Malignant Characteristics of Oral Squamous Cell Carcinoma Possibly Via the Epithelium Proliferation/Differentiation Program; Authors - Tian-Tian Xu
human	WB	Int J Mol Sci; Water-Soluble Fullerenol C60(OH)36 toward Effective Anti-Air Pollution Induced by Urban Particulate Matter in HaCaT Cell.; Authors - Chiang-Wen Lee

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with minimal background.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Human Keratinocytes

Involucrin Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:55328-1-AP)