Involucrin Polyclonal antibody

Involucrin Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55328-1-AP

Synonyms

involucrin, IVL



Tested Applications

Positive WB detected inRaji cells, NIH/3T3 cells
Positive IHC detected inhuman skin cancer tissue, human thymus tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55328-1-AP targets Involucrin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name involucrin
Calculated Molecular Weight 69 kDa
Observed Molecular Weight 80-90 kDa, 56-70 kDa
GenBank Accession NumberNM_005547
Gene Symbol Involucrin
Gene ID (NCBI) 3713
RRIDAB_11182270
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Involucrin is a protein precursor of the epidermal cornified envelope that is assembled in the outermost layers of the epidermis. Involucrin expression is restricted to the suprabasal epidermal layers (spinous and granular layers) during normal keratinocyte differentiation and is a useful marker of terminal differentiation. The predicted MW of involucrin is 68 kDa, while different reports provided variable results ranging from 90-140 kDa. (PMID: 11099111, 1503502)

Protocols

Product Specific Protocols
WB protocol for Involucrin antibody 55328-1-APDownload protocol
IHC protocol for Involucrin antibody 55328-1-APDownload protocol
IF protocol for Involucrin antibody 55328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Sci Rep

Loss-of-function Mutation in PMVK Causes Autosomal Dominant Disseminated Superficial Porokeratosis.

Authors - Jiuxiang Wang
mouseWB

J Ethnopharmacol

The Water Extracts from the Oil Cakes of Prinsepia utilis Repair the Epidermal Barrier via Up-Regulating Corneocyte Envelope-Proteins, Lipid Synthases, and Tight Junction Proteins

Authors - Ying Tu
humanWB

J Eur Acad Dermatol Venereol

Differentially expressed proteins identified by TMT proteomics analysis in children with verrucous epidermal naevi.

Authors - T Yuan
humanWB

Oncotarget

Transcriptional suppression of microRNA-27a contributes to laryngeal cancer differentiation via GSK-3β-involved Wnt/β-catenin pathway.

Authors - Sheng Chen
humanWB

Front Oncol

Cystatin-B Negatively Regulates the Malignant Characteristics of Oral Squamous Cell Carcinoma Possibly Via the Epithelium Proliferation/Differentiation Program

Authors - Tian-Tian Xu
humanWB

Int J Mol Sci

Water-Soluble Fullerenol C60(OH)36 toward Effective Anti-Air Pollution Induced by Urban Particulate Matter in HaCaT Cell.

Authors - Chiang-Wen Lee

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with minimal background.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human Keratinocytes
Involucrin Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:55328-1-AP)