Anticorps Polyclonal de lapin anti-SLC25A1

• Phare
• Validé par KD/KO

SLC25A1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

25

Avis (2)

N° de cat : 15235-1-AP

Synonymes

CIC, Citrate transport protein, CTP, SLC20A3, SLC25A1, Tricarboxylate carrier protein

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Galerie de données de validation

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  WB analysis using 15235-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15235-1-AP (SLC25A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15235-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15235-1-AP (SLC25A1 Antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15235-1-AP

  IP result of anti-SLC25A1 (IP:15235-1-AP, 2ug; Detection:15235-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human breast cancer using 15235-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15235-1-AP (SLC25A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15235-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15235-1-AP (SLC25A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15235-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15235-1-AP (SLC25A1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15235-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15235-1-AP (SLC25A1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15235-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15235-1-AP (SLC25A1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15235-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15235-1-AP (SLC25A1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 15235-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15235-1-AP (SLC25A1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15235-1-AP

  Immunofluorescent analysis of HepG2 cells using 15235-1-AP (SLC25A1 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules NCI-H1299
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du poumon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 23 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

15235-1-AP cible SLC25A1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SLC25A1 Protéine recombinante Ag7352
• Nom complet: solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 30-34 kDa
• Numéro d’acquisition GenBank: BC004980
• Symbole du gène: SLC25A1
• Identification du gène (NCBI): 6576
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)

Publications

Species	Application	Title
mouse	WB	Nature; A non-canonical tricarboxylic acid cycle underlies cellular identity.; Authors - Paige K Arnold
		Circulation; Glycolytic Switch Is Required for Transdifferentiation to Endothelial Lineage.; Authors - Li Lai
mouse	WB,IP	Nat Commun; IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice.; Authors - Weiwei Liu
mouse	WB	EMBO J; Proline biosynthesis is a vent for TGFβ-induced mitochondrial redox stress.; Authors - Simon Schwörer
human	WB	Am J Hum Genet; Deficiency in SLC25A1, encoding the mitochondrial citrate carrier, causes combined D-2- and L-2-hydroxyglutaric aciduria.; Authors - Benjamin Nota
human,mouse	WB,IHC	Cell Death Differ; Inhibition of the mitochondrial citrate carrier, Slc25a1, reverts steatosis, glucose intolerance, and inflammation in preclinical models of NAFLD/NASH.; Authors - Mingjun Tan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Bruna (Verified Customer) (03-05-2022)

This ab works very well for WB in human and mouse cell lines. Very strong and specific band (tested with KO cells lines).

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MEFs/U2OS

FH

Chunling (Verified Customer) (12-06-2018)

This antibody works great for western blot and Immunofluorescence. It is very specific with no non specific bands in western.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: kidney cancer cells

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