ACLY Polyclonal antibody

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  WB analysis using 15421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ATP citrate lyase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15421-1-AP

  WB result of ACLY antibody (15421-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ACLY transfected A549 cells.

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  WB analysis using 15421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ACLY antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15421-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15421-1-AP (ATP citrate lyase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15421-1-AP

  IP result of anti-ACLY (IP:15421-1-AP, 4ug; Detection:15421-1-AP 1:8000) with HeLa cells lysate 1320 ug.

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  IHC staining of human prostate cancer using 15421-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15421-1-AP (ACLY antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 15421-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15421-1-AP (ACLY antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 15421-1-AP

  Immunofluorescent analysis of HepG2 cells, using ACLY antibody 15421-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 15421-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ACLY (15421-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• Featured Product
• KD/KO Validated

ACLY Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 15421-1-AP

Publications(82)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

ATP citrate lyase, ACL, pro-S-, EC:2.3.3.8, ATP-citrate synthase

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Tested Applications

• Positive WB detected in: L02 cells, A549 cells, HeLa cells, mouse liver tissue, rat liver tissue, Jurkat cells, K-562 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 17 publications below
• WB: See 78 publications below
• IHC: See 7 publications below
• IF: See 10 publications below
• IP: See 4 publications below
• CoIP: See 4 publications below

Product Information

15421-1-AP targets ACLY in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACLY fusion protein Ag7709
• Full Name: ATP citrate lyase
• Calculated Molecular Weight: 121 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC006195
• Gene Symbol: ACLY
• Gene ID (NCBI): 47
• RRID: AB_2223741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P53396
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Nature; Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate.; Authors - Steven Zhao
mouse	WB,IF	Nature; Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.; Authors - Philipp Mews
human,mouse	WB	Cell; Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals.; Authors - Xiaojing Liu ; KO Validated
mouse	WB	Cell Metab; High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate; Authors - Peng Zhou
mouse	WB	Mol Cell; Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation; Authors - Sophie Trefely ; KO Validated
human	WB	Mol Cell; Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination.; Authors - Sharanya Sivanand ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Uthra (Verified Customer) (01-28-2021)

Excellent for IHC of the liver tissue under PFA fixation. The dilution of primary antibody I used 1:200.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Liver

ACLY Antibody Immunohistochemistry validation (1:200 dilution) in Liver (Cat no:15421-1-AP)

FH

K (Verified Customer) (12-20-2020)

This antibody is working good for WB (1:1000) and IHC (1:200)

• Applications: Western Blot, Immunohistochemistry

ACLY Antibody Western Blot,Immunohistochemistry validation ( dilution) in (Cat no:15421-1-AP)