Validation Data Gallery
|Positive WB detected in||L02 cells, A549 cells, HeLa cells, mouse liver tissue, rat liver tissue, Jurkat cells, K-562 cells, MCF-7 cells|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15421-1-AP targets ACLY in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACLY fusion protein Ag7709|
|Full Name||ATP citrate lyase|
|Calculated molecular weight||121 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC006195|
|Gene ID (NCBI)||47|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.
|Product Specific Protocols|
|WB protocol for ACLY antibody 15421-1-AP||Download protocol|
|IHC protocol for ACLY antibody 15421-1-AP||Download protocol|
|IF protocol for ACLY antibody 15421-1-AP||Download protocol|
|IP protocol for ACLY antibody 15421-1-AP||Download protocol|
|FC protocol for ACLY antibody 15421-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate.
Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.
Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals.
Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation
Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination.
Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Uthra (Verified Customer) (01-28-2021)
Excellent for IHC of the liver tissue under PFA fixation. The dilution of primary antibody I used 1:200.
K (Verified Customer) (12-20-2020)
This antibody is working good for WB (1:1000) and IHC (1:200)