Anticorps Polyclonal de lapin anti-SIRT3

• Phare
• Validé par KD/KO

SIRT3 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

110

Avis (1)

N° de cat : 10099-1-AP

Synonymes

hSIRT3, SIR2 like protein 3, SIR2L3, SIRT3

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Galerie de données de validation

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  WB analysis using 10099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10099-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 10099-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10099-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10099-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10099-1-AP (SIRT3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HT-29 using 10099-1-AP

  IP result of anti-SIRT3 (IP:10099-1-AP, 4ug; Detection:10099-1-AP 1:3000) with HT-29 cells lysate 1080 ug.

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  IHC staining of human stomach using 10099-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10099-1-AP (SIRT3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 10099-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10099-1-AP (SIRT3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HT-29, cellules HEK-293, cellules HepG2, tissu cardiaque de rat, tissu cardiaque de souris, tissu de muscle squelettique de souris, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de souris, tissu splénique humain
• Résultats positifs en IP: cellules HT-29,
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 19 publications below
• WB: See 107 publications below
• IP: See 2 publications below
• CoIP: See 4 publications below

Informations sur le produit

10099-1-AP cible SIRT3 dans les applications de WB, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: SIRT3 Protéine recombinante Ag0148
• Nom complet: sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC001042
• Symbole du gène: SIRT3
• Identification du gène (NCBI): 23410
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. full-length hSIRT3 (44 kDa) is an inert protein and that it is activated inside the mitochondria following deletion of 142 amino acids of the N-terminal segment. This processed form of SIRT3 is approximately 28 kDa and possesses deacetylase activity. The full SIRT3 is a 44 kDa protein and is processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
mouse	WB,CoIP	Redox Biol; Adiponectin/AdiopR1 signaling prevents mitochondrial dysfunction and oxidative injury after traumatic brain injury in a SIRT3 dependent manner.; Authors - Shenghao Zhang
rat	WB	J Pharm Anal; Cornus officinalis with high pressure wine steaming enhanced anti-hepatic fibrosis: Possible through SIRT3-AMPK axis; Authors - Xin Han
mouse	WB	Biomed Pharmacother; Cyclovirobuxine D alleviates aldosterone-induced myocardial hypertrophy by protecting mitochondrial function depending on the mutual regulation of Nrf2-SIRT3; Authors - Xueting Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain

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